Calcium channel, Ca++ mobilization, and mechanical reactivity of estrogen- and progesterone-treated rat uterus.
Calcium channel, Ca++ mobilization, and mechanical reactivity of estrogen- and progesterone-treated rat uterus.
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雌激素和孕激素处理的大鼠子宫的钙通道、钙动员和机械反应性。
DOI：
10.1254/jjp.41.47
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
J. Ando
中科院分区：
文献类型：
--
作者：
K. Ishii;T. Kano;J. Ando
在雌激素和孕酮处理的大鼠子宫标本中，观察了尼群地平结合、高K+和高Ca+引起的收缩以及维拉帕米对高K+引起的收缩的抑制作用。在尼群地平粗膜部分的结合实验中，单独用雌激素或雌激素+孕酮处理可显著降低Kd，Bmax变化不大。在钙离子耗竭、高K+含量的培养液中，只有孕酮和雌激素-孕酮处理的子宫产生收缩。雌激素、雌激素-黄体酮和雌激素+黄体酮处理的子宫显示出最大收缩所需的钙离子浓度降低。在雌激素和雌激素+孕酮处理的子宫中，维拉帕米的量效曲线平行左移。这些结果表明，雌激素可能增加钙通道的亲和力，增加钙离子跨膜内流，导致收缩增强，而孕酮可能增加细胞内钙离子的储存。
Properties of [3H]nitrendipine binding, high K+- and Ca++-induced contractions and the inhibition of high K+-induced contractions by verapamil were investigated in the uterine preparations isolated from rats treated with estrogen or progesterone or both. In [3H]nitrendipine binding experiments using crude membrane fractions, treatment with estrogen alone or estrogen+progesterone significantly lowered the KD; There was very little change in the Bmax. In the Ca++-depleted, high K+-containing medium, only the progesterone-, and estrogen----progesterone-treated uteri produced contractions. The estrogen-, estrogen----progesterone-, and estrogen+progesterone-treated uteri showed decreases in concentrations of Ca++ required for the maximal contractions. In the estrogen- and estrogen+progesterone-treated uteri, the dose-response curves by verapamil were shifted to the left in a parallel manner. These findings suggest that estrogen appeared to increase the affinity of calcium channels and increase transmembrane influx of Ca++, leading to enhancement of contractions, whereas progesterone might increase the Ca++ storage in the intracellular sites.
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Venter,JC;Fraser,CM;Schaber,JS;Jung,CY;Bolger,G;Triggle,DJ
通讯作者：
Triggle,DJ
Ca 通道拮抗剂 [3H]尼群地平与豚鼠回肠平滑肌结合的表征。
DOI：
--
发表时间：
1983
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Bolger,GT;Gengo,P;Klockowski,R;Luchowski,E;Siegel,H;Janis,RA;Triggle,AM;Triggle,DJ
通讯作者：
Triggle,DJ
[3H]尼群地平标记的钙通道拮抗剂结合位点的组织异质性。
影响因子：
3.6
作者：
Gould,RJ;Murphy,KM;Snyder,SH
通讯作者：
Snyder,SH