Comparative assessment of antibacterial activity of uvaria chamae parts
Comparative assessment of antibacterial activity of uvaria chamae parts
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紫薇各部位抗菌活性的比较评价
DOI：
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发表时间：
2010
影响因子：
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通讯作者：
Omonefe O Omofuma
中科院分区：
文献类型：
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作者：
B. Oluremi;M. Osungunna;Omonefe O Omofuma
采用标准琼脂扩散法，分别在50、100、150、200、250 mg/ml条件下，评价了chamae根、茎、叶甲醇提取物对耐甲氧西林金黄色葡萄球菌（MRSA）、金黄色葡萄球菌、大肠杆菌、克雷伯氏菌、变形杆菌以及大肠杆菌ATCC 25922、金黄色葡萄球菌ATCC 25923和铜绿假单胞菌ATCC27853的抑菌活性。初步的植物化学筛选显示其含有生物碱、单宁、黄酮类化合物、心苷和氰苷。总的来说，茎皮提取物对所有被试生物的生长都有抑制作用，而叶提取物的抑菌活性最低。甲醇茎提取物对大肠杆菌、MRSA、克雷伯氏菌、变形杆菌、大肠杆菌ATCC 25922、金黄色葡萄球菌ATCC 25923和铜绿假单胞菌ATCC 27853的MIC分别为5.62、12.59、200、35.48、28.18、5.62和19.95 mg/ml，而不含克雷伯氏菌的根提取物的MIC分别为31.62、10.0、3.55、2.82、12.59和39.81 mg/ml。甲醇叶提取物对铜绿假单胞菌的MIC值分别为200、19.95、250、250、250和200 mg/ml，对铜绿假单胞菌无MIC值。
Methanolic extracts of the root, stem and leaf of Uvaria chamae were evaluated for their antibacterial activity against Methicillin-Resistant Staphylococcus aureus(MRSA), Staphylococcus aureus, Escherichia coli, Klebsiella spp, Proteus spp, and typed strains E. coli ATCC 25922, S. aureus ATCC 25923 and Pseudomonas aeruginosa ATCC27853 using standard agar diffusion method at 50, 100, 150, 200 and 250 mg/ml. Preliminary phytochemical screening revealed the presence of alkaloids, tannins, flavonoids, cardiac glycosides and cyanogenic glycosides. In general, the stem bark extract inhibited the growth of all the tested organisms with leaf extract showing the least antibacterial activity. The MIC of methanolic stem extract on E. coli, MRSA, Klebsiella spp, Proteus spp, E. coli ATCC 25922, S. aureusATCC 25923 and P. aeruginosa ATCC 27853 were 5.62, 12.59, 200, 35.48, 28.18, 5.62 and 19.95 mg/ml respectively while that of root extract with the exclusion ofKlebsiella spp were 31.62, 10.0, 3.55 2.82, 12.59 and 39.81 mg/ml respectively. Methanolic leaf extract was found to have MIC 200, 19.95, 250, 250, 250 and 200 mg/ml respectively with no MIC value against P. aeruginosa.
Key words: Methicillin-resistant, phytochemical screening, alkaloids, cyanogenic, flavonoids.