NAD-Malic Enzyme Affects Nitrogen Fixing Activity of Bradyrhizobium japonicum USDA 110 Bacteroids in Soybean Nodules.
NAD-Malic Enzyme Affects Nitrogen Fixing Activity of Bradyrhizobium japonicum USDA 110 Bacteroids in Soybean Nodules.
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NAD-苹果酸酶影响大豆根瘤中日本慢生根瘤菌 USDA 110 类杆菌的固氮活性。
DOI：
10.1264/jsme2.23.215
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发表时间：
2008
影响因子：
2.2
通讯作者：
S. Tajima
中科院分区：
文献类型：
--
作者：
T. Dao;M. Nomura;R. Hamaguchi;Kensuke Kato;Manabu Itakura;K. Minamisawa;Suphawat Sinsuwongwat;H. T. Le;T. Kaneko;S. Tabata;S. Tajima
NAD（+）依赖的苹果酸酶（DME）是苜蓿中华根瘤菌（Sinorhizobium meliloti）类菌体固氮酶活性的关键酶。通过构建dme突变体获得了在慢生型大豆根瘤菌USDA 110中具有类似作用的遗传证据。接种dme突变体的大豆植株没有表现出延迟结瘤，但形成了小根瘤，并表现出明显的缺氮症状。接种dme突变体后14天和28天的根瘤数和每个根瘤的乙炔还原活性（作为干重值）与接种野生型B的植物相当。日本的而地上部干重和单位根瘤的乙炔还原活性则下降到约10%。野生型B的植物中的值的30%。山茱萸测定了根瘤中蔗糖和有机酸（苹果酸、琥珀酸、乙酸、α-酮戊二酸和乳酸）的含量。蔗糖，苹果酸和α-酮戊二酸的量增加接种与DME突变体，这表明减少DME和固氮酶活性的类杆菌导致这些呼吸代谢产物的消耗减少的结节。结果表明，B.日本根瘤菌类杆菌在根瘤代谢中起作用并支持固氮。
The NAD(+)-dependent malic enzyme (DME) has been reported to play a key role supporting nitrogenase activity in bacteroids of Sinorhizobium meliloti. Genetic evidence for a similar role in Bradyrhizobium japonicum USDA110 was obtained by constructing a dme mutant. Soybean plants inoculated with a dme mutant did not show delayed nodulation, but formed small root nodules and exhibited significant nitrogen-deficiency symptoms. Nodule numbers and the acetylene reducting activity per nodule as a dry weight value 14 and 28 days after inoculation with the dme mutant were comparable to those of plants inoculated with wild-type B. japonicum. However, shoot dry weight and acetylene reducting activity per nodule decreased to ca. 30% of the values in plants with wild-type B. japonicum. The sucrose and organic acid (malate, succinate, acetate, α-ketoglutarate and lactate) contents of the nodules were investigated. Amounts of sucrose, malate and a-ketoglutarate increased on inoculation with the dme mutant, suggesting that the decreased DME and nitrogenase activities in the bacteroids resulted in a reduction in the consumption of these respiratory metabolites by the nodules. The data suggest that the DME activity of B. japonicum bacteroids plays a role in nodule metabolism and supports nitrogen fixation.
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Minamisawa, K.：“具有高拷贝数的重复序列 RSα 的新慢生根瘤菌”Appl. Environ. 64. 1845-1851 (1998)。
DOI：
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发表时间：
--
期刊：
影响因子：
--
作者：
通讯作者：
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影响因子：
4.1
作者：
Kaneko, T;Nakamura, Y;Tabata, S
通讯作者：
Tabata, S
影响因子：
4.2
作者：
R. Sameshima;Tsuyoshi Isawa;M. Sadowsky;T. Hamada;H. Kasai;A. Shutsrirung;H. Mitsui;K. Minamisawa
通讯作者：
R. Sameshima;Tsuyoshi Isawa;M. Sadowsky;T. Hamada;H. Kasai;A. Shutsrirung;H. Mitsui;K. Minamisawa
影响因子：
4.9
作者：
Nomura, M;Mai, HT;Tajima, S
通讯作者：
Tajima, S