Contribution of spinal galectin-3 to acute herpetic allodynia in mice
Contribution of spinal galectin-3 to acute herpetic allodynia in mice
复制标题
DOI：
10.1016/j.pain.2011.11.022
复制
发表时间：
2012-03-01
期刊：
影响因子：
7.4
通讯作者：
Kuraishi, Yasushi
中科院分区：
文献类型：
--
作者：
Takasaki, Ichiro;Taniguchi, Kana;Kuraishi, Yasushi
为了确定参与疱疹性疼痛的内源性因素，我们对接种单纯疱疹病毒1型诱导的疱疹性异常疼痛小鼠的脊髓进行了全基因组微阵列分析，结果显示半乳糖凝集素3（一种β-半乳糖苷结合凝集素）具有明显的诱导作用。因此，我们研究了半乳糖凝集素-3在疱疹性异常性疼痛中的作用。半乳糖凝集素-3 mRNA和蛋白的表达水平增加的时间模式类似的疱疹性异常性疼痛。半乳糖凝集素-3表达细胞主要定位于浅层背角，圆形，巨噬细胞/小胶质细胞标志物Iba-1和F4/80呈阳性。在深背角，有Iba-1阳性细胞的分支和粗壮的过程，这是阴性的galectin-3。在浅层背角中，有许多CD 3阳性T细胞，但大多数半乳糖凝集素-3表达细胞对CD 3呈阴性。表达半乳凝素-3的细胞对神经元标记物NeuN和星形胶质细胞标记物胶质纤维酸性蛋白抗体呈阴性。缺乏半乳糖凝集素-3可显著降低疱疹性异常性疼痛，但对带状疱疹样皮肤病变无影响。鞘内注射半乳糖凝集素-3在幼稚小鼠中产生机械性异常性疼痛，鞘内注射抗半乳糖凝集素-3抗体显著降低疱疹性异常性疼痛。本研究结果表明，半乳糖凝集素-3在浸润的巨噬细胞和/或常驻小胶质细胞在脊髓背角有助于疱疹性异常性疼痛。Galectin-3可能成为治疗带状疱疹相关疼痛的新靶点。(C)2011年国际疼痛研究协会。Elsevier B. V.出版，保留所有权利。
To identify endogenous factors involved in herpetic pain, we performed genome-wide microarray analysis of the spinal cord of mice that suffered from herpetic allodynia induced by inoculation with herpes simplex virus type 1, which revealed marked induction of galectin-3, a beta-galactoside-binding lectin. Therefore, we investigated the role of galectin-3 in herpetic allodynia. The expression levels of galectin-3 mRNA and protein were increased with a temporal pattern similar to that of herpetic allodynia. Galectin-3-expressing cells were mainly localized in the superficial dorsal horn, round in shape, and positive for the macrophage/microglia markers Iba-1 and F4/80. In the deep dorsal horn, there were Iba-1-positive cells with ramified and stout processes, which were negative for galectin-3. In the superficial dorsal horn, there were many CD3-positive T cells, but most of the galectin-3-expressing cells were negative for CD3. Galectin-3-expressing cells were negative for the neuronal marker NeuN and the astrocyte marker glial fibrillary acidic protein antibody. Deficiency in galectin-3 markedly reduced herpetic allodynia, without showing an effect on herpes zoster-like skin lesions. Intrathecal injection of galectin-3 produced mechanical allodynia in naive mice, and intrathecal injections of anti-galectin-3 antibodies significantly reduced herpetic allodynia. The present results suggest that galectin-3 in infiltrating macrophages and/or resident microglia in the spinal dorsal horn contributes to herpetic allodynia. Galectin-3 may be a new therapeutic target for the treatment of herpes zoster-associated pain. (C) 2011 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.