4NQO CARCINOGENESIS - A MOUSE MODEL OF ORAL CAVITY SQUAMOUS-CELL CARCINOMA
4NQO CARCINOGENESIS - A MOUSE MODEL OF ORAL CAVITY SQUAMOUS-CELL CARCINOMA
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DOI：
10.1002/hed.2880160506
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发表时间：
1994-09-01
影响因子：
2.9
通讯作者：
HENDLER, FJ
中科院分区：
文献类型：
--
作者：
HAWKINS, BL;HENIFORD, BW;HENDLER, FJ
背景需要一种口腔癌发生的小鼠模型来研究恶性转化的分子方面。4-硝基喹啉-1-氧化物（4 NQO）是一种水溶性致癌物，可在啮齿类动物中产生鳞状细胞癌。协议的目的是调查肿瘤转化的时间方面。将4 NQO局部应用于小鼠腭长达16周。24 ~ 49周处死小鼠，观察其生长情况。病变范围从乳腺癌到中分化浸润性鳞状细胞癌（SCC）。病变的严重程度与治疗持续时间和观察时间相对应。没有对4 NQO的炎症反应的肉眼或显微镜证据。实验组小鼠病变为局灶性，以正常黏膜为主。4 NQO可靠地产生癌前病变和恶性口腔病变，其在形态学和组织学上模拟人头颈癌。病变在4 NQO暴露后很长时间才发展，并且没有炎症反应。因此，该模型应该是有用的肿瘤转化的分子分析。(C)John Wiley & Sons，Inc.
Background. A murine model of oral cavity carcinogenesis is needed to study the molecular aspects of malignant transformation. 4-Nitroquinoline-1-oxide (4NQO), a water-soluble carcinogen, produces squamous cell carcinoma in rodents. Protocols were designed to investigate the temporal aspects of neoplastic transformation.Methods. 4NQO was applied topically to mouse palates for up to 16 weeks. Mice were observed and killed from 24 to 49 weeks,Results. A spectrum of lesions ranging from atypia to moderately differentiated invasive squamous cell carcinoma (SCC) was produced. The severity of the lesions corresponded to the duration of treatment and the length of observation. There was no gross or microscopic evidence of an inflammatory reaction to 4NQO. The lesions were focal and normal mucosa predominated in the treated mice.Conclusion. 4NQO reliably produced preneoplastic and malignant oral cavity lesions, which morphologically and histologically mimic human head and neck cancer. Lesions develop long after 4NQO exposure and without an inflammatory response. Thus, the model should be useful for molecular analysis of neoplastic transformation. (C) 1994 John Wiley & Sons, Inc.