Paenibacillus caui sp. nov., a nitrogen-fixing species isolated from the rhizosphere soil of a peach tree
Paenibacillus caui sp. nov., a nitrogen-fixing species isolated from the rhizosphere soil of a peach tree
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椰树类芽孢杆菌 sp.
DOI：
10.1099/ijsem.0.005216
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发表时间：
2022
影响因子：
2.8
通讯作者：
Sanfeng Chen
中科院分区：
文献类型：
--
作者：
Qing X. Li;Yin-Zhao Zuo;Gao Miao;Sanfeng Chen
从河北省邯郸市桃树根际土壤中分离到一株固氮、芽内形成、活动、杆状兼性好氧细菌81-11T。从16S rRNA基因序列比较，该菌株与腓尼基类芽孢杆菌DSM 27463T（96.9%）和粪类芽孢杆菌DSM 23593T（96.7%）亲缘关系最密切。菌株81-11T的基因组大小为4.4 Mb，包含4879个预测基因，DNA G+C含量为50.0 mol%。新分离物与近缘种P. phoenicis DSM 27463T和P. faecis DSM 23593T型株基因组序列的平均核苷酸同源性分别为71.8%和72.1%。细胞脂肪酸主要为前iso-C15: 0（47.8%）、iso-C16: 0（15.5%）和iso-C15: 0（13.0%）。甲基萘醌-7是主要的呼吸醌类。极性脂类含有磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、氨基磷脂、氨基糖、未知极性脂类和未知氨基磷酸糖脂。基于系统发育、基因组学和表型特征，菌株81-11T被归类为Paenibacillus caui sp. 11 .属的一个新种。菌株类型为81-11T （=JCM 34618T=CGMCC 1.18907T）。
A nitrogen-fixing, endospore-forming, motile, rod-shaped, facultative aerobic bacterium, designated 81-11T, was isolated from rhizosphere soil of a peach tree collected from Handan, Hebei, PR China. From the comparison of 16S rRNA gene sequence, the strain is most closely related to Paenibacillus phoenicis DSM 27463T (96.9 %) and Paenibacillus faecis DSM 23593T (96.7 %). The genome size of strain 81-11T was 4.4 Mb, comprising 4879 predicted genes with a DNA G+C content of 50.0 mol%. The average nucleotide identity values of genome sequences between the novel isolate and the type strains of related species P. phoenicis DSM 27463T and P. faecis DSM 23593T were 71.8 and 72.1 %, respectively. The major cellular fatty acids were anteiso-C15 : 0(47.8 %), iso-C16 : 0 (15.5 %) and iso-C15 : 0 (13.0 %). Menaquinone-7 was the major respiratory quinone. The polar lipids contained phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, aminophospholipid, aminoglycopid, unknown polar lipids and unidentified aminophosphoglycolipid. Based on phylogenetic, genomic and phenotypic characteristics, strain 81-11T was classified as a novel species within the genus Paenibacillus, for which the name Paenibacillus caui sp. nov. is proposed. The type strain of Paenibacillus caui is 81-11T (=JCM 34618T=CGMCC 1.18907T).