Handheld tunable focus confocal microscope utilizing a double-clad fiber coupler for in vivo imaging of oral epithelium.
Handheld tunable focus confocal microscope utilizing a double-clad fiber coupler for in vivo imaging of oral epithelium.
复制标题
DOI：
10.1117/1.jbo.22.5.056008
复制
发表时间：
2017-05-01
影响因子：
3.5
通讯作者：
Maitland KC
中科院分区：
文献类型：
--
作者：
Olsovsky C;Hinsdale T;Cuenca R;Cheng YL;Wright JM;Rees TD;Jo JA;Maitland KC
采用双包层光纤耦合器和电调聚焦透镜的反射式共焦显微内镜用于口腔粘膜的成像。该仪器设计轻巧，坚固耐用，适合临床使用。当探针尖端与组织接触时，可调透镜允许轴向扫描通过上皮。图像以每秒6.6帧的速度采集，视场直径高达。在口腔中广泛的正常部位的体内成像证明了手持式探头的可访问性。诊断为炎症和发育异常的临床病变的体内成像说明了反射共聚焦显微内镜成像与病理相关的细胞变化的能力。
A reflectance confocal endomicroscope with double-clad fiber coupler and electrically tunable focus lens is applied to imaging of the oral mucosa. The instrument is designed to be lightweight and robust for clinical use. The tunable lens allows axial scanning through in the epithelium when the probe tip is placed in contact with tissue. Images are acquired at 6.6 frames per second with a field of view diameter up to . In vivo imaging of a wide range of normal sites in the oral cavity demonstrates the accessibility of the handheld probe. In vivo imaging of clinical lesions diagnosed as inflammation and dysplasia illustrates the ability of reflectance confocal endomicroscopy to image cellular changes associated with pathology.