A cleavable cross-linking reaction for protein carboxyl groups.
A cleavable cross-linking reaction for protein carboxyl groups.
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蛋白质羧基的可裂解交联反应。
DOI:
10.1111/j.1399-3011.1983.tb02078.x
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发表时间:
1983
期刊:
影响因子:
--
通讯作者:
Renthal,R
中科院分区:
文献类型:
--
作者:
Renthal,R
l‐(+)‐tartaric acid dihydrazide was coupled to the purple membrane fromHalobacterium halobiumwith a water soluble carbodiimide. Gel electrophoresis analysis of the product revealed the formation of bacteriorhodopsin dimers, trimers and higher polymers. Most of the cross‐links were removed by treatment with papain, demonstrating involvement of the carboxyl‐rich carboxyl‐terminal region of bacteriorhodopsin in the reaction. The cross‐links were cleavable by periodate oxidation.
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