Characteristics of tissue-engineered cartilage on macroporous biodegradable PLGA scaffold
Characteristics of tissue-engineered cartilage on macroporous biodegradable PLGA scaffold
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DOI：
10.1097/01.mlg.0000233521.49393.0d
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发表时间：
2006-10-01
期刊：
影响因子：
2.6
通讯作者：
Ko, Ye-Jeung
中科院分区：
文献类型：
--
作者：
Baek, Chung-Hwan;Ko, Ye-Jeung
背景资料：本研究的目的是建立软骨细胞在可生物降解的聚D，L-乳酸-羟基乙酸共聚物（PLGA）支架上的体内培养，并分析重建软骨的特性。研究方法：在植入前，将在聚甲基丙烯酸羟乙酯（poly-HEMA）包被的培养皿上生长的体外培养的软骨细胞接种到PLGA支架上以制备细胞-聚合物结构。将一种细胞支架构建体小心地植入裸鼠的皮下袋中，并将另一种无细胞支架植入同一裸鼠的相对侧作为对照。形态学，生物化学和免疫组织化学。在裸鼠中收获8周和16周后检查PLGA构建体内培养的细胞的特性。结果如下：在该体内研究中，新软骨在8周的时间内开始产生，并且新软骨形成在4个月内完成，具有原始支架的精确尺寸。所有外植体均显示正常陷窝内存活软骨细胞的不规则形状和成熟软骨基质，并且它们对II型胶原免疫染色呈阳性。结论：在PLGA支架上构建的组织工程化软骨具有软骨组织的生物化学特征，其表型和形态与天然软骨相似，具有软骨细胞特异性。
Background: The purpose of this study was to establish in vivo culture of chondrocytes on biodegradable, poly-D,L-lactic-co-glycolic acid (PLGA) scaffolds and to analyze the characteristics of the reconstructed cartilage. Methods: In vitro cultured chondrocytes that were grown on a polyhydroxyethyl methacrylate (poly-HEMA) coated dish were seeded onto the PLGA scaffolds to make a cell-polymer construct before implantation. One cell scaffold construct was carefully implanted in the subcutaneous pocket of a nude mouse and another cell-free scaffold was implanted in the opposite side of the same nude mouse as the control. Morphologic, biochemical, and immunohistochemical. characteristics of cells cultured within the PLGA constructs were examined after 8 weeks and 16 weeks of harvesting in the nude mouse. Results: New cartilage began to be generated in the period of 8 weeks and the neocartilage formation was accomplished in 4 months with the exact dimensions of the original scaffold in this in vivo study. All the explants showed the irregular shape of viable chondrocytes within normal lacunae and a mature cartilaginous matrix, and they positively immunostained for collagen type II. Conclusion: The new tissue-engineered cartilage in vivo on PLGA scaffolds displayed the biochemical characteristics of cartilage tissue, and it showed chondrocyte-specific phenotypes and morphology that were similar to the native cartilage.