Handheld high-throughput plasmonic biosensor using computational on-chip imaging
Handheld high-throughput plasmonic biosensor using computational on-chip imaging
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DOI：
10.1038/lsa.2014.3
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发表时间：
2014-01-01
影响因子：
19.4
通讯作者：
Altug, Hatice
中科院分区：
文献类型：
--
作者：
Cetin, Arif E.;Coskun, Ahmet F.;Altug, Hatice
我们展示了一种手持式芯片上生物传感技术，它使用等离子体微阵列与无透镜计算成像系统相结合，以多路复用和高通量筛选生物分子相互作用，用于医疗保健应用和资源有限的环境。该生物传感装置重量为60g，高7.5厘米，重量轻，现场可携带，利用紧凑的光电传感器阵列记录等离子体纳米结构在均匀照明下的衍射图，该衍射图由单个发光二极管调谐到纳米孔的等离子体模式。采用与无透镜计算成像相结合的灵敏等离子体阵列设计，我们展示了蛋白质层厚度降至3纳米的生物分子的无标记和定量检测。我们的芯片成像平台集成了大规模等离子体微阵列，能够在同一平台上同时检测多种生物分子浓度的蛋白质单层和双层。在这款手持设备中，我们还采用了一种基于迭代相位恢复的图像重建方法，该方法能够在同一等离子芯片上对高度多路复用的传感器阵列进行数字成像，使该方法特别适用于现场环境中的高通量诊断应用。
We demonstrate a handheld on-chip biosensing technology that employs plasmonic microarrays coupled with a lens-free computational imaging system towards multiplexed and high-throughput screening of biomolecular interactions for point-of-care applications and resource-limited settings. This lightweight and field-portable biosensing device, weighing 60 g and 7.5 cm tall, utilizes a compact optoelectronic sensor array to record the diffraction patterns of plasmonic nanostructures under uniform illumination by a single-light emitting diode tuned to the plasmonic mode of the nanoapertures. Employing a sensitive plasmonic array design that is combined with lens-free computational imaging, we demonstrate label-free and quantitative detection of biomolecules with a protein layer thickness down to 3 nm. Integrating large-scale plasmonic microarrays, our on-chip imaging platform enables simultaneous detection of protein mono-and bilayers on the same platform over a wide range of biomolecule concentrations. In this handheld device, we also employ an iterative phase retrieval-based image reconstruction method, which offers the ability to digitally image a highly multiplexed array of sensors on the same plasmonic chip, making this approach especially suitable for high-throughput diagnostic applications in field settings.