Follicle deviation and ovulatory capacity in Bos indicus heifers
Follicle deviation and ovulatory capacity in Bos indicus heifers
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DOI：
10.1016/j.theriogenology.2008.01.001
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发表时间：
2008-04-15
期刊：
影响因子：
2.8
通讯作者：
Baruselli, P. S.
中科院分区：
文献类型：
--
作者：
Gimenes, L. U.;Sa Filho, M. F.;Baruselli, P. S.
试验一采用观察法和计算法测定了东北牛（Bos indicus）母牛从排卵到偏离的时间间隔以及偏离时的优势卵泡（DF）和最大次级卵泡（SF）直径。从排卵（第0天）至第5天，每12小时对小母牛（n = 12）进行一次超声检查。两种方法的偏离时间、偏离时DF和最大SF的直径无显著性差异（P > 0.05）。总体而言，偏离发生在2.5 +/- 0.2 d（平均值+/- S.E.M.）排卵后，DF和最大SF偏离时的直径分别为6.2 ± 0.2和5.9 ± 0.2 mm。实验2旨在确定B中DF获得排卵能力的大小。印度小母牛每24小时通过超声检查监测29头小母牛，从排卵直到DF达到直径7.0-8.4 mm（n = 9）、8.5-10.0 mm（n = 10）或>10.0 mm（n = 10）。当时，小母牛用25 mg pLH处理，并每12 h通过超声监测一次，持续48 h。3/9、8/10和9/10头母牛分别出现排卵（P < 0.05）。总之，观察和计算的确定卵泡偏离开始的方法之间没有显著差异。排卵后2.5天，当DF达到6.2 mm时发生偏差，DF小至7.0 mm即可获得排卵能力。（c）2008 Elsevier Inc. All rights reserved.
The objectives of Experiment I were to determine the interval from ovulation to deviation, and diameter of the dominant follicle (DF) and largest subordinate follicle (SF) at deviation in Nelore (Bos indicus) heifers by two methods (observed and calculated). Heifers (n = 12) were examined ultrasonographically every 12 h from ovulation (Day 0) to Day 5. The time of deviation and diameter of the DF and largest SF at deviation did not differ (P > 0.05) between observed and calculated methods. Overall, deviation occurred 2.5 +/- 0.2 d (mean +/- S.E.M.) after ovulation, and diameters for DF and largest SF at deviation were 6.2 +/- 0.2 and 5.9 +/- 0.2 mm, respectively. Experiment 2 was designed to determine the size at which the DF acquires ovulatory capacity in B. indicus heifers. Twenty-nine heifers were monitored every 24 h by ultrasonography, from ovulation until the DF reached diameters of 7.0-8.4 mm (n = 9), 8.5-10.0 mm (n = 10), or >10.0 mm (n = 10). At that time, heifers were treated with 25 mg of pLH and monitored by ultrasonography every 12 h for 48 h. Ovulation occurred in 3 of 9, 8 of 10, and 9 of 10 heifers, respectively (P < 0.05). In summary, there was no significant difference between observed and calculated methods of determining the beginning of follicle deviation. Deviation occurred 2.5 d after ovulation when the DF reached 6.2 mm, and ovulatory capacity was acquired by DF as small as 7.0 mm. (c) 2008 Elsevier Inc. All rights reserved.