2-(Phthalimino)ethyl Trifluoromethanesulfonate as a Highly Reactive Ultraviolet-Labeling Agent for Carboxylic Acids in High Performance Liquid Chromatography
2-(Phthalimino)ethyl Trifluoromethanesulfonate as a Highly Reactive Ultraviolet-Labeling Agent for Carboxylic Acids in High Performance Liquid Chromatography
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2-(邻苯二甲胺基)乙基三氟甲磺酸盐作为高效液相色谱中羧酸的高反应性紫外标记剂
DOI：
10.2116/analsci.6.49
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发表时间：
1990
影响因子：
1.6
通讯作者：
T. Shono
中科院分区：
文献类型：
--
作者：
Y. Yasaka;M. Tanaka;Takashi Matsumoto;J. Katakawa;T. Tetsumi;T. Shono
本文介绍了三氟甲磺酸2-（邻苯二甲亚胺基）乙酯在制备用于高效液相色谱分光光度检测的羧酸2-（邻苯二甲亚胺基）乙酯衍生物中的应用。该试剂通过N -羟乙基邻苯二甲酰亚胺与三氟甲磺酸酐在吡啶存在下在二氯甲烷中反应以60%收率制备，并且在冰箱中至少稳定1年。羧酸钾盐（10^-5 M）与10倍当量过量的试剂在乙腈中在室温下在18-冠-6作为催化剂存在下在10分钟内反应完全。该试剂衍生化方法已成功地应用于小鼠脑中某些羧酸的测定。
The use of 2-(phthalimino)ethyl trifluoromethanesulfonate in the preparation of carboxylic acid 2-(phthalimino)ethyl ester derivatives for specrophotometric detection in high performance liquid chromatography is described. The reagent is prepared in 60% yield by the reaction of N -hydroxyethylphthalimide with trifluoromethanesulfonic anhydride in the presence of pyridine in dichloromethane and is stable at least for 1 year in a refrigerator. Reactions of carboxylate potassium salts (10^−5 M) with a 10-fold equivalent excess amount of the reagent in acetonitrile at room temperature proceed to completion within 10 min in the presence of 18-crown-6 as a catalyst. The derivatization procedure with this reagent has been successfully applied to the determination of some carboxylic acids in mouse brain.