Development of an amperometric L-lactate biosensor based on L-lactate oxidase immobilized through silica sol-gel film on multi-walled carbon nanotubes/platinum nanoparticle modified glassy carbon electrode
Development of an amperometric L-lactate biosensor based on L-lactate oxidase immobilized through silica sol-gel film on multi-walled carbon nanotubes/platinum nanoparticle modified glassy carbon electrode
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DOI：
10.1016/j.msec.2007.04.033
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发表时间：
2008-08-01
期刊：
影响因子：
--
通讯作者：
Chen, Qiang
中科院分区：
文献类型：
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作者：
Huang, Jiadong;Li, Jing;Chen, Qiang
铂纳米粒子 (Ptnano) 与多壁碳纳米管 (MWCNT) 结合使用来制造灵敏度增强的电化学 L-乳酸生物传感器。 MWCNTs和Ptnano的复合膜分散在玻碳电极（GCE）的表面。 L-乳酸氧化酶（LOD）通过吸附固定在MWCNTs/Ptnano/GCE 表面。所得LOD/MWCNTs/Ptnano电极上覆盖一层薄薄的溶胶凝胶，以避免测定中LOD的损失并提高抗干扰能力。此外，溶胶-凝胶微环境有助于增强稳定性和渗透选择性。循环伏安法结果表明，MWCNTs/Ptnano催化剂表现出比MWCNTs更高的性能。在外加电位0.5 V、pH 6.4、室温的优化条件下，该生物传感器表现出测定范围大(0.2-2.0 mM)、响应时间短(5 s内)、高灵敏度(6.36 mu A mM(-1))和良好的稳定性(4周后仍保持90%)。所制造的生物传感器实际上具有良好的抗干扰选择性。本生物传感器测量的全血样品的结果与分光光度法测量的结果非常吻合。 (c) 2007 Elsevier B.V. 保留所有权利。
Platinum nanoparticles (Ptnano) were used in combination with multi-walled carbon nanotubes (MWCNTs) for fabricating sensitivity-enhanced electrochemical L-lactate biosensor. The composite film of MWCNTs and Ptnano was dispersed on the surface of the glassy carbon electrode (GCE). L-lactate oxidase (LOD) was immobilized on MWCNTs/Ptnano/GCE surface by adsorption. The resulting LOD/MWCNTs/Ptnano electrode was covered by a thin layer of sol-gel to avoid the loss of LOD in determination and to improve the anti-interferent ability. Moreover, the sol-gel microenviroment contributes to both intensified stability and permselectivity. The cyclic voltammetry results indicated that MWCNTs/Ptnano catalyst displayed a higher performance than MWCNTs. Under the optimized conditions of applied potential 0.5 V, pH 6.4, room temperature, the proposed biosensor showed a large determination range (0.2-2.0 mM), a short response time (within 5 s), a high sensitivity (6.36 mu A mM(-1)) and good stability (90% remains after 4 weeks). The fabricated biosensor had practically good selectivity against interferences. The results for whole blood samples measured by the present biosensor showed a good agreement with those measured by spectrophotometric method. (c) 2007 Elsevier B.V. All rights reserved.