Metabolic and behavioral parameters of mice with reduced expression of Syndecan-1
Metabolic and behavioral parameters of mice with reduced expression of Syndecan-1
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DOI：
10.1371/journal.pone.0219604
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发表时间：
2019-07-12
期刊：
影响因子：
3.7
通讯作者：
Baston-Buest, Dunja Maria
中科院分区：
文献类型：
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作者：
Gougoula, Christina;Bielfeld, Alexandra Petra;Baston-Buest, Dunja Maria
能量平衡对所有物种都是至关重要的。配体-受体的相互作用调节了基于能量状态的生殖和新陈代谢等身体活动。这样的受体是硫酸乙酰肝素蛋白多糖，特别是Syndecans家族。因此，我们研究了杂合子Syndecan 1小鼠(Sdc1(+/-))和相应野生型小鼠Sdc1表达降低的代谢参数的差异。Sdc1(+/-)小鼠体重减轻，尽管它们的瘦素水平增加，皮质酮水平降低。此外，他们的食物和水摄入量也增加了。伴随而来的是脂肪组织减少，脂肪细胞变小，从而脂肪细胞密度增加。为了详细分析新陈代谢，使用了自动化的Pheno-Master系统，该系统可以连续和不受干扰地记录食物和水的摄入量、能量消耗和运动。体重较低的原因是与对照组相比，这些动物的能量消耗更高。此外，雌性Sdc1(+/-)小鼠表现出更多的运动活动。在器官方面，Sdc1(+/-)小鼠的肠道更重、更长，但在细胞水平上没有观察到差异。这些发现与Sdc1(+/-)和野生型胚胎在其他基因型受体雌性受体中的正常交配或相反的胚胎移植无关。在这里，我们发现Sdc1的表达减少导致胎儿和母体一侧的代谢改变，这可能在人类妊娠病理中观察到的生长受限和缺乏Sdc1的小鼠中起作用。
Energy balance is essential for all species. Ligand-receptor interactions mediate processes that regulate body activities like reproduction and metabolism based on the energy status. Such receptors are the heparan sulfate proteoglycans and specifically the family of syndecans. Therefore we investigated the differences of metabolic parameters of heterozygous Syndecan 1 mice (Sdc1(+/-)) with reduced expression of Sdc1 and the corresponding wild type mice. Sdc1(+/-) mice have a reduced body weight although they show increased leptin and decreased corticosterone levels. Furthermore, their food and water intake is increased. This is accompanied with less adipose tissue, smaller adipocytes and thus an increased density of adipocytes. For the detailed analysis of the metabolism the automated Pheno-Master system has been used, which allowed continuous and undisturbed recording of food and water intake, energy expenditure and movement. The reason for the lower body weight was the higher energy expenditure of these animals compared to controls. Additionally, female Sdc1(+/-) mice showed an increased locomotor activity. Referring to organs, the intestine in Sdc1(+/-) mice was heavier and longer, but no differences at the cellular level could be observed. These findings were independent of normal mating or vice versa embryo transfers of Sdc1(+/-) and wild type embryos in recipient females of the other genotype. Herein we showed that the reduced expression of Sdc1 led to an altered metabolism on fetal as well as on maternal side, which may play a role in the growth restriction observed in human pregnancy pathologies and in mice lacking Sdc1.