Characterization of alphasatellites associated with monopartite begomovirus/betasatellite complexes in Yunnan, China.
Characterization of alphasatellites associated with monopartite begomovirus/betasatellite complexes in Yunnan, China.
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DOI：
10.1186/1743-422x-7-178
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发表时间：
2010-08-03
期刊：
影响因子：
4.8
通讯作者：
Zhou X
中科院分区：
文献类型：
--
作者：
Xie Y;Wu P;Liu P;Gong H;Zhou X
Alphasatellites是单链分子，与单分裂的菜豆花叶病毒/betasatellite复合物相关。在中国云南的菜豆花叶病毒病植物样品中鉴定出Alphasatellites。所有含有β星的样品也含有β星，但只有一些含有β星的样品含有β星。对33个小卫星进行了测序，它们的范围从1360到1376个核苷酸。所有的小卫星都含有3个保守的特征：一个单一的开放阅读框架（Rep），一个保守的发夹结构，和一个富含腺嘌呤（A-丰富）的区域。根据全序列的系统发育树，将这些小卫星分为3种类型，只有一种例外。1型与中国番茄黄化曲叶病毒（TYLCCNV）/中国番茄黄化曲叶β卫星（TYLCCNB）复合体有关。2型与烟草曲茎病毒（Tobacco curly shoot virus，TbCSV）/烟草曲茎β卫星（Tobacco curly shoot betasatellite，TbCSB）复合体有关。3型为TbCSV/胜红蓟黄脉β星（AYVB）复合体。在每个类型内，核苷酸序列同一性范围为83.4%至99.7%，而类型之间的同一性为63.4%至81.3%。记录了与菜豆病毒/β卫星复合体相关的α卫星混合感染。我们的研究结果验证，β-星只与菜豆病毒/β-星复合物。从中国云南省分离的33株甜菜小卫星可分为3种类型，每种类型都与一种特定的菜豆花叶病毒/甜菜小卫星复合体相关。小卫星分子的混合感染可能并不罕见。
Alphasatellites are single-stranded molecules that are associated with monopartite begomovirus/betasatellite complexes. Alphasatellites were identified in begomovirus-infected plant samples in Yunnan, China. All samples that contained alphasatellites also contained betasatellites, but only some samples that contained betasatellites contained alphasatellites. Thirty-three alphasatellites were sequenced, and they ranged from 1360 to 1376 nucleotides. All alphasatellites contain 3 conserved features: a single open reading frame (Rep), a conserved hairpin structure, and an adenine-rich (A-rich) region. On the basis of the phylogenetic tree of the complete nucleotide sequences, the alphasatellites were divided into 3 types with one exception. Type 1 was associated with Tomato yellow leaf curl China virus (TYLCCNV)/Tomato yellow leaf curl China betasatellite (TYLCCNB) complex. Type 2 was associated with Tobacco curly shoot virus (TbCSV)/Tobacco curly shoot betasatellite (TbCSB) complex. Type 3 was associated with TbCSV/Ageratum yellow vein betasatellite (AYVB) complex. Within each type, nucleotide sequence identity ranged from 83.4 to 99.7%, while 63.4-81.3% identity was found between types. Mixed infections of alphasatellites associated with begomovirus/betasatellite complexes were documented. Our results validate that alphasatellites are only associated with begomovirus/betasatellite complexes. Thirty-three sequenced alphasatellites isolated from Yunnan Province, China were divided into 3 types--each associated with a specific begomovirus/betasatellite complex. Mix-infections of alphasatellite molecules may not be unusual.