Identification of chelicerate neuropeptides using bioinformatics of publicly accessible expressed sequence tags
Identification of chelicerate neuropeptides using bioinformatics of publicly accessible expressed sequence tags
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DOI：
10.1016/j.ygcen.2010.09.018
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发表时间：
2011-01-01
影响因子：
2.7
通讯作者：
Lenz, Petra H.
中科院分区：
文献类型：
--
作者：
Christie, Andrew E.;Nolan, Daniel H.;Lenz, Petra H.
虽然许多研究都集中在节肢动物神经肽的鉴定上，但大多数研究都是在六足动物或甲壳类动物身上进行的，目前对螯角动物的神经肽知之甚少。在这里，公开可访问的表达序列标签（ESTs）被挖掘出推定的螯合神经肽编码转录本：通过在线肽预测程序和已知同种异构体的同源性推断出由ESTs编码的肽。使用该策略鉴定了58个est，代表8个肽家族/亚家族。值得注意的是，预测了第一个真正的螯合c型化抑素，pQIRYHQCYFNPISCF，来自荨麻疹叶螨，以及预测了一种新的化抑素CC肽，GEGKMFWRCYFNAVSCF，来自蜱的变种Amblyomma和Mesobuthus gibbosus。此外，还鉴定出了真正的甲壳类心脏活性肽（CCAP）、几种属于甲壳类高血糖激素/离子转运肽超家族的肽、几种类似降钙素的利尿激素/利尿激素31家族的肽，以及几种类似fmrfamily的肽，特别是神经肽F （NPF）和短神经肽F亚家族的肽。据我们所知，在T. urticae中首次鉴定出CCAP和NPF。此外，从jendeki蝎子和Loxosceles beta蜘蛛中发现了几种新的orcokinins，并预测了此前未知的s.j endeki SIFamide、ESRNPPLNGSMFamide和ESKNPPLNGSMFamide亚型。总的来说，我们研究中的数据扩展了已知螯合神经肽的目录，并为未来在这些动物中进行螯合神经肽的生理学研究奠定了基础。(C) 2010爱思唯尔公司版权所有。
While numerous investigations have focused on the identification of neuropeptides in arthropods, most have been conducted on members of the Hexapoda or Crustacea, and little is currently known about those in the Chelicerata. Here, publicly accessible expressed sequence tags (ESTs) were mined for putative chelicerate neuropeptide-encoding transcripts: the peptides encoded by the ESTs were deduced using online peptide prediction programs and homology to known isoforms. Fifty-eight ESTs representing eight peptide families/subfamilies were identified using this strategy. Of note was the prediction of the first authentic chelicerate C-type allatostatin, pQIRYHQCYFNPISCF, from the mite Tetranychus urticae, as well as the prediction a novel allatostatin CC peptide, GEGKMFWRCYFNAVSCF, from both the tick Amblyomma variegatum and the scorpion Mesobuthus gibbosus. Also identified from T. urticae were authentic crustacean cardioactive peptide (CCAP), several peptides belonging to the crustacean hyperglycemic hormone/ion transport peptide superfamily, members of the calcitonin-like diuretic hormone/diuretic hormone 31 family, and several FMRFamide-like peptides, specifically members of the neuropeptide F (NPF) and short neuropeptide F subfamilies. To the best of our knowledge the identifications of CCAP and NPF in T. urticae are the first for the Chelicerata. In addition, several novel orcokinins were identified from the scorpion Scorpiops jendeki and the spider Loxosceles beta: in S. jendeki previously unknown isoforms of SIFamide, ESRNPPLNGSMFamide and ESKNPPLNGSMFamide, were also predicted. Taken collectively, the data presented in our study expand the catalog of known chelicerate neuropeptides and provide a foundation for future physiological studies of them in these animals. (C) 2010 Elsevier Inc. All rights reserved.