Whole-genome analysis of two-component signal transduction genes in fungal pathogens
Whole-genome analysis of two-component signal transduction genes in fungal pathogens
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DOI：
10.1128/ec.2.6.1151-1161.2003
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发表时间：
2003-12-01
期刊：
影响因子：
--
通讯作者：
Turgeon, BG
中科院分区：
文献类型：
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作者：
Catlett, NL;Yoder, OC;Turgeon, BG
双组分磷酸传递系统最低限度由组氨酸激酶(HK)组分和响应调节(RR)组分组成，组氨酸激酶(HK)组分响应环境刺激而自动磷酸化，响应调节器(RR)组分传递信号，导致转录激活等输出，或丝裂原激活的蛋白激酶级联反应。酿酒酵母、庞氏裂殖酵母和白色念珠菌的基因组分别编码一个、三个和三个HKS。相比之下，丝状子囊菌Neuropora crassa、Cochliobolus Heterostrophus(Bipolaris Maydis)、串珠赤霉(Gibberella Moniliformis)和灰霉病菌(Botrytis Cinerea)的基因组序列编码一个广泛的双组分信号蛋白家族。推定的香港证券交易所分为11个类别。这些纲中的大多数在所研究的每个丝状子囊菌物种中都有表现。其中几个类别在真菌病原体中的流行程度明显高于粗毛沙门氏菌，这表明这些类别包含致病力所需的副对数。尽管丝状子囊菌中的HKS数量多于酵母菌，但所有的子囊菌含有几乎相同的下游组氨酸磷酸转移蛋白和RR蛋白，这表明HKS之间存在广泛的串扰或冗余。
Two-component phosphorelay systems are minimally comprised of a histidine kinase (HK) component, which autophosphorylates in response to an environmental stimulus, and a response regulator (RR) component, which transmits the signal, resulting in an output such as activation of transcription, or of a mitogen-activated protein kinase cascade. The genomes of the yeasts Saccharomyces cerevisiae, Schizosaccharomyces pombe, and Candida albicans encode one, three, and three HKs, respectively. In contrast, the genome sequences of the filamentous ascomycetes Neurospora crassa, Cochliobolus heterostrophus (Bipolaris maydis), Gibberella moniliformis (Fusarium verticillioides), and Botryotinia fuckeliana (Botrytis cinerea) encode an extensive family of two-component signaling proteins. The putative HKs fall into 11 classes. Most of these classes are represented in each filamentous ascomycete species examined. A few of these classes are significantly more prevalent in the fungal pathogens than in the saprobe N. crassa, suggesting that these groups contain paralogs required for virulence. Despite the larger numbers of HKs in filamentous ascomycetes than in yeasts, all of the ascomycetes contain virtually the same downstream histidine phosphotransfer proteins and RR proteins, suggesting extensive cross talk or redundancy among HKs.