An extracellular copper/zinc superoxide dismutase (ecCuZnSOD) from Chinese mitten crab, Eriocheir sinensis and its relationship with Spiroplasma eriocheiris
An extracellular copper/zinc superoxide dismutase (ecCuZnSOD) from Chinese mitten crab, Eriocheir sinensis and its relationship with Spiroplasma eriocheiris
复制标题
中华绒螯蟹胞外铜/锌超氧化物歧化酶(ecCuZnSOD)及其与绒毛螺原体的关系
DOI：
10.1016/j.aquaculture.2011.08.014
复制
发表时间：
2011-10-19
期刊：
影响因子：
4.5
通讯作者：
Wang, Wen
中科院分区：
文献类型：
--
作者：
Meng, Qingguo;Du, Jie;Wang, Wen
从中华绒螯蟹血细胞中克隆了一个细胞外铜锌超氧化物歧化酶（ecCuZnSOD）基因。ecCuZnSOD cDNA全长891 bp，开放阅读框（ORF）长度为624 bp，编码信号肽序列的24个残基，共208个氨基酸。成熟蛋白的分子量（184 aa）为21.76 kDa， pi值为6.02。氨基酸负责结合Cu （His90、92、109和171）和Zn （His109、117和126）以及Asp 129，这两种半胱氨酸（95和189）形成二硫键。两个CuZnSOD签名（GFHVHEWGVDGS）从88到99，（GNAGGRVACCTI）从189到200。中华对虾ecCuZnSOD的氨基酸序列与罗氏沼虾ecCuZnSOD的相似度分别为75%和58.7%。血浆鼠毛螺旋体是中华绒螯蟹震颤病的一种新型病原体。本文还研究了中华绒螯蟹ecCuZnSOD与绒螯蟹的关系。血细胞中ecCuZnSOD转录量在1 h内显著增加，24 h后恢复到初始值，接种鸡毛弧菌192 h后增加。ecCuZnSOD转录本在服服毛弧菌1 h后显著升高，提示在短时间内诱导了蟹的免疫系统。192 h后，ecCuZnSOD转录量增加，同时出现了蟹的震颤症状，ecCuZnSOD的诱导与寄主抗鼠毛弧菌的免疫应答有关。(C) 2011 Elsevier B.V.版权所有
An extracellular copper-zinc superoxide dismutase (ecCuZnSOD) gene was cloned from hemocytes of Chinese mitten crab, Eriocheir sinensis. The ecCuZnSOD cDNA consists of 891 bp with an open reading frame (ORF) of 624 bp that encodes 208 amino acids (aa) with 24 residues of a signal peptide sequence. The predicted molecular mass of the mature protein (184 aa) is 21.76 kDa with an estimated pi of 6.02. Amino acids are responsible for binding Cu (His90, 92, 109, and 171) and Zn (His109, 117, and 126, and Asp 129), two cysteines (95 and 189) that form a disulfide bond. two CuZnSOD signatures (GFHVHEWGVDGS) from 88 to 99 and (GNAGGRVACCTI) from 189 to 200. The deduced amino acid sequence of E. sinensis ecCuZnSOD showed similarity of 75% and 58.7% with the ecCuZnSOD of giant freshwater prawn Macrobrachium rosenbergii and mud crab Scylla serrata, respectively. Spiro plasma eriocheiris is a novel pathogen of tremor disease of E. sinensis. This paper also studied the relationship between E. sinensis ecCuZnSOD and S. eriocheiris. The ecCuZnSOD transcript in hemocytes significantly increased in 1 h, had returned to the original value by 24 h, and increased after 192 h by the S. eriocheiris inoculation. The ecCuZnSOD transcript increased significantly at 1 h after S. eriocheiris injection indicating induction of the immune system of the crab within a short time. After 192 h the ecCuZnSOD transcript increased, meanwhile the tremor symptom of the crab also appeared, induction of the ecCuZnSOD has relation with the immune response of hosts to resist S. eriocheiris. (C) 2011 Elsevier B.V. All rights reserved.