Quantitative detection of sapoviruses in wastewater and river water in Japan
Quantitative detection of sapoviruses in wastewater and river water in Japan
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DOI：
10.1111/j.1472-765x.2008.02330.x
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发表时间：
2008-03-01
影响因子：
2.4
通讯作者：
Ohgaki, S.
中科院分区：
文献类型：
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作者：
Haramoto, E.;Katayama, H.;Ohgaki, S.
目的：定量检测日本某污水处理厂和某河流中的血吸虫病毒。方法与结果：在1年的时间里，每个月收集一个污水处理厂的进水和出水样本和河流样本。用HA电负性过滤器对水样进行病毒浓缩，然后用实时PCR对病毒进行定量。进水中腺病毒的浓度为每升2.8 × 10(3)至1.3 × 10(5)份，在冬季较高。12个流出物样本中有7个（58%）呈沙病毒阳性，从多摩川河沿岸3个地点收集的36个河水样本中有23个（64%）呈沙病毒阳性。结论：即使在非流行期，该河流中也有大量的腺病毒，表明全年都有散发和无症状感染。在流行期和冬季，沙病毒排放到河流中的浓度不断增加。研究意义和影响：这是第一个在水生环境中定量检测萨帕病毒的研究。
Aims: To detect sapoviruses at a wastewater treatment plant (WWTP) and in a river in Japan, quantitatively.Methods and Results: Influent and effluent samples at a WWTP and river water samples were collected monthly for 1 year. The water samples were subjected to virus concentration using an HA electronegative filter, followed by quantification of sapoviruses using real-time PCR. The concentration of sapoviruses in influent ranged from 2.8 x 10(3) to 1.3 x 10(5) copies per litre, showing a higher value in winter. Seven (58%) of 12 effluent samples were positive for sapoviruses, as were 23 (64%) of 36 river water samples collected from three sites along the Tamagawa River.Conclusions: Sapoviruses were abundant in the influent even in the nonepidemic period, suggesting that sporadic and asymptomatic infections occur throughout the year. Increasing concentration of sapoviruses was discharged into the river during the epidemic period winter.Significance and Impact of the Study: This is the first study demonstrating the quantitative detection of sapoviruses in aquatic environments.