Overexpression of Arabidopsis Dehydration-Responsive Element-Binding Protein 2C Confers Tolerance to Oxidative Stress
Overexpression of Arabidopsis Dehydration-Responsive Element-Binding Protein 2C Confers Tolerance to Oxidative Stress
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DOI：
10.1007/s10059-012-2188-2
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发表时间：
2012-02-01
影响因子：
3.8
通讯作者：
Lim, Chae Oh
中科院分区：
文献类型：
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作者：
Hwang, Jung Eun;Lim, Chan Ju;Lim, Chae Oh
脱水反应元件结合蛋白(DREB)调节植物对环境胁迫的反应。在本研究中，DREB2C类基因的转录被超氧阴离子增殖剂甲基紫精(MV)诱导。在光照条件下，通过离子渗漏和叶绿素荧光检测，过量表达DREB2C的转基因植株的氧化胁迫耐受性显著高于野生型植株。在DREB2C过表达中诱导了几个抗坏血酸过氧化物酶(APX)基因的转录活性和APX蛋白的活性。此外，在相似的氧化胁迫条件下，过表达的植物中的过氧化氢水平低于wt植物。凝胶迁移率改变分析和瞬时激活报告实验表明，APX2的表达受热休克因子A3(HsfA3)的调控，HsfA3受DREB2C在转录水平上的调控。这些结果表明，DREB2C在提高拟南芥的氧化胁迫耐受性方面起着重要作用。
Dehydration-responsive element-binding proteins (DREBs) regulate plant responses to environmental stresses. In the current study, transcription of DREB2C, a class 2 Arabidopsis DREB, was induced by a superoxide anion propagator, methyl viologen (MV). The oxidative stress tolerance of DREB2C-overexpressing transgenic plants was significantly greater than that of wild-type plants, as measured by ion leakage and chlorophyll fluorescence under light conditions. The transcriptional activity of several ascorbate peroxidase (APX) genes as well as APX protein activity was induced in DREB2C overexpressors. Additionally, the level of H2O2 in the overexpressors was lower than in wt plants under similar oxidative stress conditions. An electrophoretic mobility shift assay and transient activator-reporter assay showed that APX2 expression was regulated by heat shock factor A3 (HsfA3) and that HsfA3 is regulated at the transcriptional level by DREB2C. These results suggest that DREB2C plays an important role in promoting oxidative stress tolerance in Arabidopsis.