IRREVERSIBLE INHIBITION OF BIOTIN TRANSPORT IN YEAST BY BIOTINYL PARA NITROPHENYL ESTER
IRREVERSIBLE INHIBITION OF BIOTIN TRANSPORT IN YEAST BY BIOTINYL PARA NITROPHENYL ESTER
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DOI：
10.1073/pnas.68.10.2604
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发表时间：
1971-01-01
影响因子：
11.1
通讯作者：
KATCHALS.E
中科院分区：
文献类型：
--
作者：
BECKER, JM;WILCHEK, M;KATCHALS.E
生物素对硝基苯酯(BNP)是生物素的一种活性酯类衍生物，它不可逆地抑制生物素在酿酒酵母中的转运。转运失活是随时间递增的，当酯的浓度低至10-7M时就会发生。在叠氮化钠的存在下，衍生物仍然有效。叠氮化钠是一种已知的阻止生物素在酵母中积累的试剂。结果表明：(A)生物素基-对-硝基苯胺和乙酰基-对-硝基苯酯不影响生物素的转运；(B)对硝基苯酯不影响赖氨酸和天冬氨酸的转运，也不影响L山梨糖的转运；(C)当细胞在相对高浓度的生物素存在下与其孵育时，可以部分地阻止其对生物素转运的失活。
Biotinyl-p-nitrophenyl ester (BNP), an active-ester derivative of biotin, irreversibly inactivates biotin transport in the yeastSaccharomyces cerevisiae. Transport inactivation is progressive with time and occurs at concentrations of the ester as low as 10-7M. In the presence of sodium azide, a reagent known to block biotin accumulation in yeast, the derivative is still effective. The specificity of inactivation by the ester is revealed by the following findings: (a) Biotinyl-p-nitroanilide and acetyl-p-nitrophenyl ester do not affect biotin transport; (b) the nitrophenyl ester does not affect the transport of lysine and aspartic acid, or that of L-sorbose; (c) inactivation of biotin transport by the ester is partially prevented when the cells are incubated with it in the presence of relatively high concentrations of biotin.