Effects of fixation time and enzymatic digestion on immunohistochemical demonstration of bromodeoxyuridine in formalin-fixed, paraffin-embedded tissue.
Effects of fixation time and enzymatic digestion on immunohistochemical demonstration of bromodeoxyuridine in formalin-fixed, paraffin-embedded tissue.
复制标题
固定时间和酶消化对福尔马林固定、石蜡包埋组织中溴脱氧尿苷免疫组织化学显示的影响。
DOI：
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发表时间：
1988
影响因子：
3.2
通讯作者：
T. Hoshino
中科院分区：
文献类型：
--
作者：
Y. Hayashi;M. Koike;M. Matsutani;T. Hoshino
我们研究了固定时间和酶消化对溴脱氧尿苷（BUdR）在离体大鼠和人胃肠道组织和人脑肿瘤的免疫组化染色的影响，这些组织在组织活检前不久静脉注射BUdR后已固定在福尔马林中。在福尔马林固定的大鼠胃肠道组织中，未用蛋白酶处理，反应产物不足以识别BUdR阳性细胞。当福尔马林固定的组织切片用蛋白酶、胃蛋白酶或胰蛋白酶处理时，得到了与乙醇固定的组织相似的结果。材料在福尔马林中固定的时间越长，鉴定BUdR标记的细胞核所需的蛋白酶孵育时间就越长。甲醛固定的人脑肿瘤标本用蛋白酶处理的BUdR标记指数与乙醇固定的组织的BUdR标记指数相当。即使在福尔马林中长时间固定的组织中也获得了足够的BUdR染色。因此，BUdR标记指数可以在福尔马林中储存的临床材料中进行回顾性测定。
We studied the effects of fixation time and enzymatic digestion on immunohistochemical staining for bromodeoxyuridine (BUdR) in excised rat and human gastrointestinal tissues and human brain tumors which had been fixed in formalin after intravenous administration of BUdR shortly before biopsy of tissue. In formalin-fixed rat gastrointestinal tissues not treated with proteinase, the reaction products were insufficient to identify BUdR-positive cells. Results similar to those in ethanol-fixed tissue were obtained when formalin-fixed tissue sections were treated with protease, pepsin, or trypsin. The longer the material had been fixed in formalin, the longer the incubation in proteinase required to identify BUdR-labeled nuclei. The BUdR labeling indices of formalin-fixed human brain tumor specimens treated with protease were comparable to those of ethanol-fixed tissues. Sufficient BUdR staining was obtained even in tissues fixed in formalin for prolonged periods. Therefore, the BUdR labeling index can be determined retrospectively in clinical materials stored in formalin.
DOI：
10.1073/pnas.80.18.5573
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DOLBEARE, F;GRATZNER, H;GRAY, JW
通讯作者：
GRAY, JW
影响因子：
4.1
作者：
Nagashima,T;Murovic,JA;Hoshino,T;Wilson,CB;DeArmond,SJ
通讯作者：
DeArmond,SJ
改良的碘脱氧尿苷高亲和力单克隆抗体。
DOI：
10.1002/cyto.990070602
发表时间：
1986
期刊：
Cytometry
影响因子：
--
作者：
Vanderlaan,M;Watkins,B;Thomas,C;Dolbeare,F;Stanker,L
通讯作者：
Stanker,L