INTERLEUKIN-1-BETA PREVENTS THE STIMULATORY EFFECT OF TRANSFORMING GROWTH-FACTOR-BETA ON COLLAGEN GENE-EXPRESSION IN HUMAN SKIN FIBROBLASTS
INTERLEUKIN-1-BETA PREVENTS THE STIMULATORY EFFECT OF TRANSFORMING GROWTH-FACTOR-BETA ON COLLAGEN GENE-EXPRESSION IN HUMAN SKIN FIBROBLASTS
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DOI：
10.1042/bj2710827
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发表时间：
1990-11-01
影响因子：
4.1
通讯作者：
HEINONEN, T
中科院分区：
文献类型：
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作者：
HEINO, J;HEINONEN, T
转化生长因子β 1和β 2（TGF-β 1和TGF-β 2）是胶原基因表达的充分表征的强诱导剂。100 pM浓度的TGF-β 1或TGF-β 2分别使人皮肤成纤维细胞中的前α 1（I）胶原mRNA水平增加6.6倍和7.0倍，并且还增加了细胞培养基中前胶原的积累。Interleukin-1.beta.（IL-1 β）是一种炎症介质，也调节结缔组织代谢。小浓度的IL-1 β。 （0.01-1.0单位/ml）轻微增加原α 1（I）胶原mRNA水平（2.2倍）。在此，我们提供了IL-1 β.阻止TGF-β的刺激作用。对人体皮肤成纤维细胞胶原合成的影响。IL-1 β。1单位/ml的浓度足以使用100 pM-TGF-β 1刺激的细胞中原α 1（I）胶原mRNA水平保持在对照值。因此，结果表明IL-1 β。抑制由TGF-β激活的细胞中的胶原合成，而它不显著改变或甚至可能刺激非活化细胞中的胶原基因表达。
Transforming growth factors .beta.1 and .beta.2 (TGF-.beta.1 and TGF-.beta.2) are well-characterized strong inducers of collagen gene expression. A 100 pM concentration of TGF-.beta.1 or TGF-.beta.2 increases pro.alpha.1(I) collagen mRNA levels in human skin fibroblasts 6.6-fold and 7.0-fold respectively, and also increases the accumulation of procollagens in the cell culture medium. Interleukin-1.beta. (IL-1.beta.) is an inflammatory mediator which also regulates connective tissue metabolism. A small concentration of IL-1.beta. (0.01-1.0 unit/ml) slightly increases pro.alpha.1(I) collagen mRNA levels (2.2-fold). Here we provide evidence that IL-1.beta. prevents the stimulatory effect of TGFs-.beta. on collagen synthesis in human skin fibroblasts. An IL-1.beta. concentration of 1 unit/ml is enough to keep pro.alpha.1(I) collagen mRNA levels at control values in cells stimulated by 100 pM-TGF-.beta.1. Thus the results indicate that IL-1.beta. inhibits collagen synthesis in cells activated by TGFs-.beta., whereas it does not significantly change or might even stimulate collagen gene expression in non-activated cells.