High resolution genetic and physical mapping of molecular markers linked to the Phytophthora resistance gene Rps1-k in soybean
High resolution genetic and physical mapping of molecular markers linked to the Phytophthora resistance gene Rps1-k in soybean
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DOI：
10.1094/mpmi.1997.10.9.1035
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发表时间：
1997-12-01
影响因子：
3.5
通讯作者：
Bhattacharyya, MK
中科院分区：
文献类型：
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作者：
Kasuga, T;Salimath, SS;Bhattacharyya, MK
大豆对大豆疫霉引起的根腐病和茎腐病的抗性是由一系列单显性Rps基因赋予的，我们应用随机扩增多态性DNA（RAPD）和扩增片段长度多态性（AFLP）分析来分离与Rps1-k连锁的分子标记，鉴定了5个RAPD标记并紧密定位到Rps1-k的一侧，对近等基因系和获得的批量进行了AFLP分析来自 F-3 家庭。鉴定了 27 个标记，其中 19 个是抗性亲本特异的，5 个 AFLP 标记是从易感亲本扩增的，其中一个标记 TC1，定位在距离 Rps1 基因座 0.07 厘摩 (cM) 处，三个 AFLP 标记是共显性的，其中一个 CG1，定位在距离 Rps1 基因座 0.06 cM 的位置，位于其余标记的另一侧，两个源自重复序列的 RAPD、17 个 AFLP 和 14 个限制性片段长度多态性 (RFLP) 标记在 3-cM 图谱间隔内作图。这些结果表明 Rps1-k 位于基因渗入区域的末端。物理作图数据表明 Rps1-k 侧翼标记 CG1 和 TC1 可能位于 125-kb 染色体片段内。
The resistance of soybean to Phytophthora root and stem rot caused by Phytophthora sojae is conferred by a series of single-dominant Rps genes, We have applied random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) analyses to isolate molecular markers linked to Rps1-k, Five RAPD markers were identified and mapped closely to one side of Rps1-k, AFLP analysis was carried out with near isogenic lines and bulks obtained from F-3 families. Twenty-seven markers were identified, Nineteen of these were specific to the resistant parent, Five AFLP markers were amplified from the susceptible parent, One of these markers, TC1, mapped at 0.07 centimorgans (cM) from the Rps1 locus, Three AFLP markers were co-dominant, and one of these, CG1, mapped at a distance of 0.06 cM from the Rps1 locus on the opposite side of the rest of the markers, Two RAPD, 17 AFLP, and 14 restriction fragment length polymorphism (RFLP) markers originating from duplicated sequences were mapped within a 3-cM map interval, These results suggest that Rps1-k is located at the end of an introgressed region, Physical mapping data indicate that the Rps1-k-flanking markers CG1 and TC1 may be located within a 125-kb chromosomal fragment.