Sustained Vascular Endothelial Growth Factor Blockade by Antivascular Endothelial Growth Factor Antibodies Results in Nonunion in the Process of Fracture Healing in Rabbits
Sustained Vascular Endothelial Growth Factor Blockade by Antivascular Endothelial Growth Factor Antibodies Results in Nonunion in the Process of Fracture Healing in Rabbits
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DOI：
10.1097/ta.0b013e31818b4e61
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发表时间：
2009-04-01
影响因子：
--
通讯作者：
Gong, Shuigen
中科院分区：
文献类型：
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作者：
Chu, Tongwei;Liu, Yugang;Gong, Shuigen
目的：为探讨血管内皮生长因子（VEGF）在骨折愈合过程中的作用及VEGF和抗VEGF多克隆抗体对骨折愈合的影响，将105只新西兰白色兔造成左侧桡骨中段骨折，随机分为对照组、VEGF组和VEGF多克隆抗体组。分别于术后8h、24 h、72 h、1周、3周、5周、8周行单光子发射计算机断层扫描（SPECT）检测骨折部位血流量。结果：与对照组相比，VEGF组骨折端血流量在8h ~ 1周内明显增加，但两组X线片上无明显差异。VEGF多克隆抗体组骨折端血流量在各时间点均较对照组明显减少。结论：VEGF缺乏可能阻碍骨折愈合，导致骨折不愈合。
Objective: To study the role of vascular endothelial growth factor (VEGF) in the process of fracture healing and the effect of VEGF and anti-VEGF polyclonal antibody on fracture healing.Methods: One hundred and five New Zealand white rabbits were subjected to fracture of the middle part of the left radius, and were randomly divided into control, VEGF, and VEGF polyclonal antibody groups. The blood flow at the fracture site was measured by single photoemission computerized tomography after 8 hours, 24 hours, and 72 hours, and 1 weeks, 3 weeks, 5 weeks, and 8 weeks. X-ray films were taken after 1 weeks, 3 weeks, 5 weeks, and 8 weeks to observe the results of fracture healing.Results: The blood flow at the fracture site in the VEGF group significantly increased compared with the control group during 8 hours to 1 week, but no obvious difference was seen on the X-ray films between the two groups. In the VEGF polyclonal antibody group, the blood flow at the fracture sites decreased significantly at all time points compared with the control group. The fracture healing process was disturbed, and nonunion signs were seen at the fracture site.Conclusions: The lack of VEGF may impede the fracture healing process, and results in nonunion at the fracture site.