The contribution of cytochemistry and immunophenotyping to the reproducibility of the FAB classification in acute leukemia.
The contribution of cytochemistry and immunophenotyping to the reproducibility of the FAB classification in acute leukemia.
复制标题
细胞化学和免疫表型对急性白血病 FAB 分类的可重复性的贡献。
DOI：
10.1182/blood.v68.4.900.bloodjournal684900
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发表时间：
1986
期刊：
影响因子：
20.3
通讯作者：
P. Soamboonsrup
中科院分区：
文献类型：
--
作者：
G. Browman;P. Neame;P. Soamboonsrup
两名独立观察者的FAB分类的观察者内和观察者间重现性进行了评估，他们的决定是对汉密尔顿地区急性白血病患者的治疗采取行动。对瑞氏染色制备物的观察者内重现性进行了评估，瑞氏染色制备物在至少间隔2周的两个连续场合进行独立检查。第三次阅读用瑞氏染色和细胞化学数据进行，第四次阅读加入免疫表型数据。使用校正概率预期一致性（k）的统计量计算一致性，并使用考虑到分歧严重性的加权统计量。105例患者的样本可用于形态学和细胞化学评估，93例标本的免疫表型数据可用。观察者A和B的观察者内一致性分别为64.8%和70.5%，kappa值分别为0.56和0.62。观察者A和观察者B分别有37个和31个不一致读数，10例病例中，每个观察者的淋巴细胞：非淋巴细胞表型之间不一致。观察员之间的一致性为63%（k = 0.54）和72%（k = 0.65）的两个单独的读数仅瑞氏染色制备。当加入细胞化学时，繁殖率提高到89%（k = 0.86）。当除了瑞氏染色和细胞化学制剂之外还提供免疫表型信息时，一致性为99%。观察者之间的淋巴细胞：非淋巴细胞不一致性发生在9次，当瑞氏染色制备物仅可用时，4次，当添加细胞化学时;免疫表型分析未发生。这项研究表明，免疫分型，当加入到急性白血病的形态学评估，可能会大大有助于观察员之间的协议。
Intraobserver and interobserver reproducibility of the FAB classification was assessed for two independent observers whose decisions are acted on for treatment of patients with acute leukemia in the Hamilton region. Intraobserver reproducibility was assessed for Wright-stained preparations that were examined independently on two consecutive occasions at least 2 weeks apart. A third reading was performed with Wright stain and cytochemical data, and the fourth reading was done with addition of immunophenotype data. Concordance was calculated using a statistic that corrects for chance-expected agreement (k), and a weighted statistic that takes into account the seriousness of disagreements was used. Samples were available for morphological and cytochemical assessment on 105 patients, and immunophenotype data were available on 93 specimens. Intraobserver concordance was 64.8% and 70.5% for observers A and B, respectively, with kappa values of .56 and .62. There were 37 discordant readings for observer A and 31 for observer B, with each observer discordant between lymphocytic:nonlymphocytic phenotypes in ten cases. Concordance between observers was 63% (k = .54) and 72% (k = .65) for each of two separate readings for Wright-stained preparations only. Reproducibility improved to 89% (k = .86) when cytochemistry was added. When immunophenotype information was provided in addition to Wright-stained and cytochemical preparations, the agreement was 99%. Lymphocytic:nonlymphocytic discordance between observers occurred on nine occasions when Wright-stained preparations only were available and four times when cytochemistry was added; it did not occur with immunophenotyping. The study suggests that immunophenotyping, when added to morphological assessment of acute leukemia, may contribute substantially to agreement between observers.
影响因子：
11.2
作者：
J. Rowley
通讯作者：
J. Rowley