Differentiation of mouse embryonic stem cells into a defined neuronal lineage
Differentiation of mouse embryonic stem cells into a defined neuronal lineage
复制标题
DOI：
10.1038/nn1301
复制
发表时间：
2004-09-01
影响因子：
25
通讯作者：
Barde, YA
中科院分区：
文献类型：
--
作者：
Bibel, M;Richter, J;Barde, YA
虽然人们早就知道，培养的胚胎干细胞可以产生神经元，但与它们的直接前体的谱系关系仍不清楚。我们在这里报道，选择高度增殖的干细胞，然后用视黄酸处理，产生了基本上纯的前体，标记物鉴定为pax -6阳性的放射状胶质细胞。正如它们在体内所做的那样，这些细胞继续产生具有非常一致的生化和电生理特征的神经元。
Although it has long been known that cultured embryonic stem cells can generate neurons, the lineage relationships with their immediate precursors remain unclear. We report here that selection of highly proliferative stem cells followed by treatment with retinoic acid generated essentially pure precursors that markers identified as Pax-6-positive radial glial cells. As they do in vivo, these cells went on to generate neurons with remarkably uniform biochemical and electrophysiological characteristics.