A novel role for dpp in the shaping of bivalve shells revealed in a conserved molluscan developmental program
A novel role for dpp in the shaping of bivalve shells revealed in a conserved molluscan developmental program
复制标题
DOI：
10.1016/j.ydbio.2009.01.021
复制
发表时间：
2009-05-01
影响因子：
2.7
通讯作者：
Wada, Hiroshi
中科院分区：
文献类型：
--
作者：
Kin, Koryu;Kakoi, Shota;Wada, Hiroshi
在软体动物进化到双壳类的过程中，单个背壳被加倍。为了阐明这一突出的形态转变的分子发育基础，我们描述了细胞分裂和表达模式的三个基因，brachyury，enrailed，和DPP在日本棘牡蛎囊牡蛎，并检查DPP在这个物种中的功能。S. kegaki胚胎的模式与先前描述的其他双壳类物种的模式几乎相同，这表明模式本身对于双壳类身体计划的建立或维持非常重要。短尾畸形同源基因在S. kegaki（SkBra）的模式与腹足类相似，即使在单细胞水平上，尽管腹足类和双壳类的分歧很大。Engrailed和dpp以前被发现在腹足类的壳原基周围表达。与腹足类一样，S. kegaki（SkEn）在壳原基周围表达。然而，S. kegaki（SkDpp）仅在沿着背中线的细胞中表达。ZfBMP 4处理实验揭示了dpp在建立双壳类壳原基的特征形状中的重要性。(C)2009 Elsevier Inc. All rights reserved.
During the molluscan evolution leading to the bivalves, the single dorsal shell was doubled. To elucidate the molecular developmental basis underlying this prominent morphological transition, we described the cell cleavage and expression patterns of three genes, brachyury, engrailed, and dpp in the Japanese spiny oyster Saccostrea kegaki, and examined the function of dpp in this species. The cleavage pattern of the S. kegaki embryo was nearly the same as the previously described pattern of other bivalve species, suggesting that the pattern itself is highly important for the establishment or the maintenance of the bivalve body plan. The expression pattern of a brachyury homolog in S. kegaki (SkBra) was similar to the pattern in gastopods even at the single cell level despite the deep divergence of gastropods and bivalves. Engrailed and dpp were previously found to be expressed around the shell anlagen in gastropods. Like that of gastropods, an engrailed homolog in S. kegaki (SkEn) was found to be expressed around the shell anlagen. However, the dpp homolog in S. kegaki (SkDpp) was expressed only in the cells along the dorsal midline. ZfBMP4 treatment experiments revealed the importance of dpp in establishing the characteristic shape of the bivalve shell anlagen. (C) 2009 Elsevier Inc. All rights reserved.