Faster embryonic segmentation through elevated Delta-Notch signalling.
Faster embryonic segmentation through elevated Delta-Notch signalling.
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DOI：
10.1038/ncomms11861
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发表时间：
2016-06-15
影响因子：
16.6
通讯作者：
Oates AC
中科院分区：
文献类型：
--
作者：
Liao BK;Jörg DJ;Oates AC
理解生物节律的一个重要步骤是控制周期。一个多细胞的，有节奏的模式系统被称为分割时钟被认为是控制脊椎动物胚胎的身体片段，体节的顺序生产。几种遗传性功能丧失条件，包括Delta-Notch细胞间信号传导突变体，导致较慢的分割。在这里，我们产生了具有一系列拷贝数和相应增加的信号水平的DeltaD转基因斑马鱼系，并观察到更快的分割。表达最高的品系显示出改变的振荡基因表达波模式和缩短的分割期，产生具有更多、更短身体片段的胚胎。我们的研究结果揭示了Notch信号强度在不同器官系统中如何定量解释的令人惊讶的差异，并表明细胞间通信通过改变其空间模式在调节分割时钟的输出周期中的作用。 在脊椎动物中，节律模式控制着体节的形成，但是这种节律的周期如何改变还不清楚。在这里，作者在斑马鱼中生成了一个遗传模型来增加DeltaD表达，这增加了Delta-Notch信号传导的范围，从而导致更快的分割。
An important step in understanding biological rhythms is the control of period. A multicellular, rhythmic patterning system termed the segmentation clock is thought to govern the sequential production of the vertebrate embryo's body segments, the somites. Several genetic loss-of-function conditions, including the Delta-Notch intercellular signalling mutants, result in slower segmentation. Here, we generate DeltaD transgenic zebrafish lines with a range of copy numbers and correspondingly increased signalling levels, and observe faster segmentation. The highest-expressing line shows an altered oscillating gene expression wave pattern and shortened segmentation period, producing embryos with more, shorter body segments. Our results reveal surprising differences in how Notch signalling strength is quantitatively interpreted in different organ systems, and suggest a role for intercellular communication in regulating the output period of the segmentation clock by altering its spatial pattern. Rhythmic patterning governs the formation of somites in vertebrates, but how the period of such rhythms can be changed is unclear. Here, the authors generate a genetic model in zebrafish to increase DeltaD expression, which increases the range of Delta-Notch signalling, causing faster segmentation.