Photoregulated tyrosinase gene in Polyporus arcularius
Photoregulated tyrosinase gene in Polyporus arcularius
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猪苓光调节酪氨酸酶基因
DOI：
10.1007/s10267-006-0327-3
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发表时间：
2007
期刊：
影响因子：
1.4
通讯作者：
T. Morinaga
中科院分区：
文献类型：
--
作者：
S. Kanda;T. Aimi;S. Masumoto;K. Nakano;Y. Kitamoto;T. Morinaga
摘要从担子菌多孔菌中克隆到酪氨酸酶基因acttyr 1，并对其进行了结构鉴定。该基因在基因组中以单拷贝存在。Tyr 1的氨基酸序列与香菇gLeTyr的氨基酸序列非常相似。然而，Tyr 1的氨基酸序列的羧基端区域在L.香菇双孢蘑菇和这种真菌结果表明，在玉米中，tyr 1的转录受光调控;在猪笼草中，tyr 1的转录在光照条件下被激活，在黑暗条件下被灭活。这些结果表明，tyr 1是一个光调控基因调控的Dof样转录因子在养殖arcularius。虽然只在双核体中观察到酶活性，但tyr 1在双核体和单核体菌株中均被转录。因此，激活Tyr 1的前体可能需要一个翻译后加工事件，这是发育调控。
Abstracttyr1, the gene for tyrosinase, which is related to pigmentation of mycelia in dikaryotic strains, has been cloned and characterized from the basidiomycete Polyporus arcularius. The gene was present in a single copy in the genome. The putative amino acid sequence of Tyr1 was very similar to that of gLeTyr from Lentinula edodes. However, the carboxy-terminal region of the amino acid sequence of Tyr1 was variable among L. edodes, Agaricus bisporus, and this fungus. In the 5′-untranslated region near the initiation codon, a consensus sequence to the Dof1 binding site that is involved in light-regulated gene expression in maize was found. Transcription of tyr1 was photoregulated; transcription of tyr1 in P. arcularius was activated in light mycelia and inactivated in the dark mycelia. These results suggest that tyr1 is a light-regulated gene regulated by a Dof-like transcription factor in P. arcularius. Although the enzyme activity was observed only in a dikaryon, tyr1 was transcripted in both dikaryotic and monokaryotic strains. Thus, activation of the precursor of Tyr1 may require a posttranslational processing event that is developmentally regulated.
DOI：
10.1073/pnas.93.23.13096
发表时间：
1996-11-12
影响因子：
11.1
作者：
BellPedersen, D;Shinohara, ML;Dunlap, JC
通讯作者：
Dunlap, JC