Identification, Characterization, and Expression Analysis of Spondin-Like and Fasciclin-Like Genes in Neopyropia yezoensis, A Marine Red Alga
Identification, Characterization, and Expression Analysis of Spondin-Like and Fasciclin-Like Genes in Neopyropia yezoensis, A Marine Red Alga
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DOI：
10.3390/phycology2010003
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发表时间：
2022-01
期刊：
影响因子：
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通讯作者：
T. Uji;Shinnosuke Ueda;Hiroyuki Mizuta
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作者：
T. Uji;Shinnosuke Ueda;Hiroyuki Mizuta
细胞外基质（ECM）蛋白在细胞增殖和分化的调节中发挥着至关重要的作用。我们鉴定了编码 ECM 蛋白的同源基因，这些蛋白已知与红藻条斑藻动物细胞中的整合素相关。选择来自条斑鱼的 4 个编码含 spondin 结构域的蛋白 (NySPL) 的基因和 8 个编码含成束蛋白结构域的蛋白 (NyFALs) 的基因进行生物信息学和表达分析，以便深入了解 ECM 蛋白在生命周期中的作用。 NySPL 有 8 条 β 链和两个连续的 α 螺旋，与动物的 F-spondin 结构域相似。 NyFAL 具有保守的 H1 和 H2 基序以及 H1 和 H2 区域之间的 YH 基序。定量逆转录聚合酶链反应表明，NySPL1-3 和 NyFAL8 转录本在形成精子的成熟配子体中高度积累。此外，所有 NySPL 的表达均因诱导配子发生的乙烯前体 1-氨基环丙烷-1-羧酸而上调。 NyFAL1、4 在孢子体中高表达，而 NyFAL2、3、5、6 和 7 在配子体中过表达，尤其是在营养阶段。这些发现有助于未来对红藻独特生命周期中 ECM 结构的研究。
Extracellular matrix (ECM) proteins play crucial roles in the regulation of cell proliferation and differentiation. We identified homologous genes encoding ECM proteins that are known to associate with integrins in animal cells in red macroalga Neopyropia yezoensis. Four genes encoding spondin domain-containing proteins (NySPLs) and eight genes encoding fasciclin domain-containing proteins (NyFALs) from N. yezoensis were selected for bioinformatics and expression analysis in order to obtain insights into the roles of ECM proteins for the life cycle. NySPLs had eight β-strands with two contiguous α-helices, which were similar to those of the F-spondin domain of animals. NyFALs had conserved H1 and H2 motifs and a YH motif between the H1 and H2 regions. Quantitative reverse transcription polymerase chain reaction showed that NySPL1–3 and NyFAL8 transcripts were highly accumulated in mature gametophytes that formed the spermatia. Furthermore, expressions of all NySPLs were upregulated in response to the ethylene precursor 1-aminocylopropane-1-carboxylic acid that induces gametogenesis. NyFAL1, 4 were highly expressed in sporophytes, whereas NyFAL2, 3, 5, 6, and 7 were overexpressed in gametophytes, especially at the vegetative stage. These findings facilitate future research on ECM architecture in the unique life cycles of red macroalgae.