Association between Mycobacterium tuberculosis Beijing/W lineage strain infection and extrathoracic tuberculosis:: Insights from epidemiologic and clinical characterization of the three principal genetic groups of M-tuberculosis clinical isolates
Association between Mycobacterium tuberculosis Beijing/W lineage strain infection and extrathoracic tuberculosis:: Insights from epidemiologic and clinical characterization of the three principal genetic groups of M-tuberculosis clinical isolates
复制标题
DOI：
10.1128/jcm.01459-06
复制
发表时间：
2007-02-01
影响因子：
9.4
通讯作者：
Yang, Z. H.
中科院分区：
文献类型：
--
作者：
Kong, Y.;Cave, M. D.;Yang, Z. H.
根据katG基因密码子463和gyrA基因密码子95处的单核苷酸多态性，结核分枝杆菌的临床菌株可分为三个主要的遗传组。遗传群1的一个亚群，北京/W谱系，由于其在世界范围内的分布和与暴发的关联而被广泛研究。为了增加我们对M的遗传分组的临床和流行病学相关性的理解。结核分枝杆菌临床分离株与北京/W株的基因分型比较，对679株结核分枝杆菌临床分离株进行了基因分型研究。结核病，占1996年1月至2000年12月使用PCR和DNA测序在阿肯色州诊断的培养确诊的结核病病例的96.3%。我们评估了不同的M基因组感染的相关性。结核菌株和北京/W系菌株感染与患者临床和流行病学特征的关系采用卡方检验和多因素Logistic回归分析。在679个研究分离株中，676个属于三个主要遗传组之一，其中63个（9.3%）属于组1，438个（64.8%）属于组2，175个（25.9%）属于组3。在多变量logistic回归模型中调整了年龄、性别、人种/种族、人类免疫缺陷病毒血清状态和plcD基因型的潜在混杂因素后，感染北京/W谱系分离株的患者发生胸外受累的可能性几乎是感染非北京/W谱系分离株的患者的3倍（比值比[95%置信区间]，2.85 [1.33，6.12]）。因此，北京/W系菌株可能具有某些特殊的生物学特性，促进胸外结核的发展。
Clinical strains of Mycobacterium tuberculosis can be divided into three principal genetic groups based on the single-nucleotide polymorphisms at the katG gene codon 463 and the gyrA gene codon 95. One subgroup of genetic group 1, the Beijing/W lineage, has been widely studied because of its worldwide distribution and association with outbreaks. In order to increase our understanding of the clinical and epidemiological relevance of the genetic grouping of M. tuberculosis clinical strains and the Beijing/W lineage, we investigated the genetic grouping of 679 clinical isolates of M. tuberculosis, representing 96.3% of culture-confirmed tuberculosis cases diagnosed in Arkansas between January 1996 and December 2000 using PCR and DNA sequencing. We assessed the associations of infections by different genetic groups of M. tuberculosis strains and infection by the Beijing/W lineage strains with the clinical and epidemiological characteristics of the patients using chi-square tests and multivariate logistic regression analysis. Of the 679 study isolates, 676 fell into one of the three principal genetic groups, with 63 (9.3%) in group 1, 438 (64.8%) in group 2, and 175 (25.9%) in group 3. After adjusting for potential confounding of age, gender, race/ethnicity, human immunodeficiency virus serostatus, and plcD genotype in a multivariate logistic regression model, patients infected by the Beijing/W lineage isolates were nearly three times as likely as patients infected with the non-Beijing/W lineage isolates to have an extrathoracic involvement (odds ratio [95% confidence interval], 2.85 [1.33, 6.12]). Thus, the Beijing/W lineage strains may have some special biological features that facilitate the development of extrathoracic tuberculosis.