Peptide Biomarker Discovery for Identification of Methicillin-Resistant and Vancomycin-Intermediate Staphylococcus aureus Strains by MALDI-TOF
Peptide Biomarker Discovery for Identification of Methicillin-Resistant and Vancomycin-Intermediate Staphylococcus aureus Strains by MALDI-TOF
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DOI：
10.1021/ac300855z
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发表时间：
2012-07-03
影响因子：
7.4
通讯作者：
Chen, Chao-Jung
中科院分区：
文献类型：
--
作者：
Lu, Jang-Jih;Tsai, Fuu-Jen;Chen, Chao-Jung
快速鉴定社区相关（CA）耐甲氧西林金黄色葡萄球菌（MASA）、医院相关（HA）MRSA和万古霉素中间体S。金黄色葡萄球菌（VISA）对于适当治疗和及时干预暴发至关重要。在这项研究中，肽生物标志物用于快速鉴定甲氧西林耐药和万古霉素中间体S。通过基质辅助激光解吸电离飞行时间质谱法发现金黄色葡萄球菌菌株。结果表明，1774.1和1792.1 m/z峰分别对应于苯酚可溶性调制蛋白α 1和苯酚可溶性调制蛋白α 2肽，存在于大多数（95%，121/127）SCCmec IV型和V型分离株中，但仅存在于8%（15/185）SCCmec I-III型分离株中。由于SCCmec型分离株被认为是HA-MRSA，而大多数CA-MRSA分离株属于SCCmec IV和V型，因此这两种肽可以作为区分HA-MRSA和CA-MRSA分离株的标志物。1835.0和1863.0 m/z峰存在于50%（4/8）的异质性VISA和88%（14/16）的VISA分离株中。这两个峰的肽被鉴定为酰基载体蛋白的蛋白水解产物。本研究结果为建立CA-MRSA、HA-MRSA和万古霉素耐药链球菌的鉴定方法提供了可能。金黄色葡萄球菌分离物的基础上存在的这些肽。
Rapid identification of community-associated (CA) methicillin-resistant Staphylococcus aureus (MASA), hospital-associated (HA) MRSA, and vancomycin-intermediate S. aureus (VISA) is essential for proper therapy and timely intervention of outbreaks. In this study, peptide biomarkers for rapid identification of methicillin-resistant and vancomycin-intermediate S. aureus strains were discovered by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The results showed that the 1774.1 and 1792.1 m/z peaks corresponding to the phenol-soluble modulin alpha 1 and phenol-soluble modulin alpha 2 peptides, respectively, were present in the majority (95%, 121 of 127) of SCCmec types IV and V isolates, but only in 8% (15 of 185) of SCCmec types I-III isolates. Since SCCmec types isolates are recognized as HA-MRSA and most CA-MRSA isolates belong to SCCmec types IV and V, these two peptides may serve as markers for discrimination between HA-MRSA and CA-MRSA isolates. The 1835.0 and 1863.0 m/z peaks were present in 50% (4 of 8) of heterogeneous VISA and 88% (14 of 16) of VISA isolates. The peptides of these two peaks were identified as proteolytic products of the acyl carrier protein. The results of this study provide the possibility to develop methods for identification of CA-MRSA, HA-MRSA, and vancomycin-resistant S. aureus isolates based on the presence of these peptides.