Cytogenetic response to coffee in Chinese hamster ovary AUXB1 cells and human peripheral lymphocytes.
Cytogenetic response to coffee in Chinese hamster ovary AUXB1 cells and human peripheral lymphocytes.
复制标题
中国仓鼠卵巢 AUXB1 细胞和人外周淋巴细胞对咖啡的细胞遗传学反应。
DOI：
10.1093/mutage/4.5.343
复制
发表时间：
1989
期刊：
影响因子：
2.7
通讯作者：
Hanna,ML
中科院分区：
文献类型：
--
作者：
Tucker,JD;Taylor,RT;Christensen,ML;Strout,CL;Hanna,ML
我们研究了三种不同品牌和三种类型的咖啡（新鲜冲泡的普通咖啡，速溶普通咖啡和新鲜冲泡的无咖啡因咖啡）在两种哺乳动物系统中的遗传毒性作用：中国仓鼠卵巢（CHO）AUXB 1细胞系和人外周血淋巴细胞。姐妹染色单体交换（SCE）和核内复制细胞（ERCs）被用作终点。根据制造商的建议制备咖啡，并在冷却后，以高达全浓度咖啡的11%的稀释度施用于培养的细胞。每种品牌和类型的咖啡在AUXB 1细胞中诱导显著水平的SCE和ERC。SCE，而不是ERCs，诱导人外周血淋巴细胞。亚硫酸氢盐与羰基化合物络合并降低其遗传毒性，显着减少了咖啡给药后发现的SCE和ERC的数量。过氧化氢酶和过氧化物酶，酶，破坏过氧化氢的活性，没有显着影响SCE和ERC的频率在AUXB 1的文化与新鲜冲泡的普通咖啡。这些实验表明，不同品牌和类型的咖啡具有足够的遗传毒性活性，可以增加SCE和ERC，其水平仅为正常消费水平的一小部分。单独的1，2-二羰基化合物和单独的过氧化物似乎并不是观察到由稀释咖啡诱导的大多数SCE和ERC的原因。
We have investigated the genotoxic effects of three different brands and three types of coffee (freshly brewed regular, instant regular and freshly brewed decaffeinated) in two mammalian systems: the Chinese hamster ovary (CHO) AUXB1 cell line and human peripheral lymphocytes. Sisterchromatid exchanges (SCEs) and endoreduplicated cells (ERCs) were used as the endpoints. Coffee was prepared according to the manufacturer's suggestions, and after cooling, administered to cultured cells at dilutions ranging up to 11% that of full-strength coffee. Each brand and type of coffee induced significant levels of SCEs and ERCs in AUXB1 cells. SCEs, but not ERCs, were induced in human peripheral lymphocytes. Bisulfite, which complexes with carbonyls and reduces their genotoxicity, significantly diminished the number of SCEs and ERCs found after administration of coffee. Catalase and peroxidase, enzymes that destroy hydrogen peroxide activity, had no significant effect upon the SCE and ERC frequencies in AUXB1 cultures treated with freshly brewed regular coffee. These experiments indicate that different brands and types of coffee have sufficient genotoxic activity to increase SCEs and ERCs at levels only a fraction of those normally consumed. 1,2-Dicarbonyls alone and peroxides alone do not appear to be responsible for the majority of SCEs and ERCs that were observed to be induced by dilute coffee.