Insight into the epigenetic landscape of a currently endogenizing gammaretrovirus in mule deer (Odocoileus hemionus).
Insight into the epigenetic landscape of a currently endogenizing gammaretrovirus in mule deer (Odocoileus hemionus).
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DOI：
10.1016/j.ygeno.2019.06.003
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发表时间：
2019
期刊：
影响因子：
4.4
通讯作者：
T. Hron;H. Fábryová;D. Elleder
中科院分区：
文献类型：
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作者：
T. Hron;H. Fábryová;D. Elleder
内源性逆转录病毒(ERV)是脊椎动物基因组的重要组成部分。它们起源于过去的逆转录病毒感染，其中一些保留了转录活性。避免ERV转录失控的关键机制是DNA甲基化介导的表观遗传沉默。尽管许多研究描述了ERV活性参与细胞过程，但对ERV的表观遗传调控仍然知之甚少。我们先前在骡鹿基因组中描述了一种宫颈内源性逆转录病毒(CrERV)。这种病毒表现出大量的插入多态，表明最近的活性。在这里，我们使用基于NGS的策略来确定CRERV在四头骡鹿中整合的甲基化模式。除了绝大多数的甲基化整合外，我们还发现了一小部分去甲基化的前病毒拷贝。这些拷贝代表了位于基因启动子附近的进化中的较老的整合。总体而言，我们的工作是首次尝试在全基因组规模上表征插入多态ERV的表观遗传学图景，并提供对其与宿主相互作用的洞察。
Endogenous retroviruses (ERVs) constitute a significant part of vertebrate genomes. They originated from past retroviral infections and some of them retain transcriptional activity. The key mechanism avoiding uncontrolled ERV transcription is DNA methylation-mediated epigenetic silencing. Despite numerous studies describing the involvement of ERV activity in cellular processes, epigenetic regulation of ERVs is still poorly understood.We previously described a cervid endogenous retrovirus (CrERV) in the mule deer genome. This virus exhibits massive insertional polymorphism, suggesting recent activity. Here we employed NGS-based strategy to determine the methylation pattern of CrERV integrations in four mule deer. Besides the vast majority of methylated integrations, we identified a tiny fraction of demethylated proviral copies. These copies represent evolutionary older integrations located near gene promoters. In general, our work is a first attempt to characterize the epigenetic landscape of insertionally polymorphic ERV on a whole-genome scale and offers insight into its interactions with a host.