EXPRESSION OF C-FOS PROTEIN BY IMMUNOHISTOCHEMICALLY IDENTIFIED OXYTOCIN NEURONS IN THE RAT HYPOTHALAMUS UPON OSMOTIC STIMULATION
EXPRESSION OF C-FOS PROTEIN BY IMMUNOHISTOCHEMICALLY IDENTIFIED OXYTOCIN NEURONS IN THE RAT HYPOTHALAMUS UPON OSMOTIC STIMULATION
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DOI：
10.1016/0006-8993(92)91342-c
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发表时间：
1992-08-14
期刊：
影响因子：
2.9
通讯作者：
BLOOM, FE
中科院分区：
文献类型：
--
作者：
GIOVANNELLI, L;SHIROMANI, PJ;BLOOM, FE
用c-fos和催产素(Oxy)双重免疫组织化学染色方法，研究了渗透压应激下大鼠下丘脑催产素能系统代谢激活的形态和时程。动物被注射ip。高渗盐水刺激后30min和6h，下丘脑室旁核(PVN)、视上核(PEV)、视上核(SON)、视前区和视交叉后区可见c-fos样免疫反应(FLI)阳性表达，而等渗盐水组这些区域几乎不表达。催产素能系统的激活在30min达到高峰，在PVN和SON在90min后以不同的速度下降。在渗透应激条件下，在SON、PEV和LSN表达Fli的Oxy神经元的最大百分比约为80%，在PVN和视前内侧区分别为60%和50%。在系统的大细胞和小细胞分裂中都发现了激活的Oxy神经元。这些数据表明，渗透应激对大鼠下丘脑Oxy核团有不同程度的激活作用。他们还表明，Oxy在中枢以及对血浆渗透压变化的体液反应中发挥了作用。
Double immunostaining for c-fos and oxytocin (OXY) was used to study the topography and time course of the metabolic activation of the hypothalamic oxytocinergic system upon osmotic stress in the male rat. Animals injected i.p. with hypertonic saline expressed c-fos-like immunoreactivity (FLI) in the paraventricular (PVN), periventricular (PEV) and supraoptic (SON) hypothalamic nuclei, and in the preoptic and retrochiasmatic regions, as early as 30 min after stimulation and up to 6 h, while these areas were mostly devoid of staining in isotonic saline-injected animals. The activation of the oxytocinergic system peaked at 30 min and declined at different rates in the PVN and in the SON after 90 min. The maximal percentage of OXY neurons expressing FLI upon osmotic stress was about 80% in the SON, PEV and LSN, 60% in the PVN and 50% in the medial preoptic area. Activated OXY neurons were found in both the magnocellular and parvocellular divisions of the system. These data show that OXY nuclei in the rat hypothalamus are differentially activated by osmotic stress. They also suggest a role of OXY in the central as well as in the humoral response to changes in plasma osmolarity.