In situ UV disinfection of a waveguide-based photobioreactor.
In situ UV disinfection of a waveguide-based photobioreactor.
复制标题
基于波导的光生物反应器的原位紫外线消毒。
DOI：
10.1021/es502812k
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发表时间：
2014
影响因子：
11.4
通讯作者：
L. T. Angenent
中科院分区：
文献类型：
--
作者：
Devin F R Doud;Aadhar Jain;S. Ahsan;D. Erickson;L. T. Angenent
已经开发出具有高表面积与体积比和最佳光管理策略的紧凑波导基光生物反应器，以实现藻类培养物内的高体积生产率。光管理策略集中于通过使用光导向波导来优化整个生物反应器体积中的阳光收集、阳光过滤和光递送。除了为藻类生长提供广谱或单色光外，这些系统还提供了通过使用杀菌紫外线（UV）光进行光生物反应器消毒的机会。在这里，我们研究了在原位，非化学紫外线处理消毒的异养污染物在紧凑的光生物反应器的功效。在对有意污染的波导光生物反应器进行UV处理后，我们将集胞藻属PCC 6803的>99%纯培养物保持超过3周的操作期。在没有UV处理的情况下，培养物仅在几天内就被污染（对照）。我们开发了一个理论模型来预测消毒效率的操作参数和生物反应器的几何形状的基础上，我们验证了它与实验结果来预测枯草芽孢杆菌孢子培养物的消毒效率。
Compact waveguide-based photobioreactors with high surface area-to-volume ratios and optimum light-management strategies have been developed to achieve high volumetric productivities within algal cultures. The light-managing strategies have focused on optimizing sunlight collection, sunlight filtration, and light delivery throughout the entire bioreactor volume by using light-directing waveguides. In addition to delivering broad-spectrum or monochromatic light for algal growth, these systems present an opportunity for advances in photobioreactor disinfection by using germicidal ultraviolet (UV) light. Here, we investigated the efficacy of in situ, nonchemical UV treatment to disinfect a heterotrophic contaminant in a compact photobioreactor. We maintained a >99% pure culture of Synechocystis sp. PCC 6803 for an operating period exceeding 3 weeks following UV treatment of an intentionally contaminated waveguide photobioreactor. Without UV treatment, the culture became contaminated within only a few days (control). We developed a theoretical model to predict disinfection efficiency based on operational parameters and bioreactor geometry, and we verified it with experimental results to predict the disinfection efficiency of a Bacillus subtilis spore culture.