THE EFFECTS OF ANESTHESIA ON OSMOTIC BLOOD-BRAIN-BARRIER DISRUPTION
THE EFFECTS OF ANESTHESIA ON OSMOTIC BLOOD-BRAIN-BARRIER DISRUPTION
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DOI：
10.1227/00006123-199002000-00014
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发表时间：
1990-02-01
期刊：
影响因子：
4.8
通讯作者：
NEUWELT, EA
中科院分区：
文献类型：
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作者：
GUMERLOCK, MK;NEUWELT, EA
为了评价不同麻醉剂对高渗性血脑屏障破坏（BBBD）的影响，Sprague-Dawley大鼠在颈动脉内输注甘露醇或生理盐水之前给予戊巴比妥（PB）、氯胺酮-甲苯噻嗪（KX）、异氟烷（IF）、甲氧呋喃（MF）或芬太尼-氟哌啶（FD）。生理监测显示，甘露醇输注的效果与生理盐水输注的效果显著不同，并与一过性心动过缓、低血压、代谢性酸中毒和脑电图抑制相关。在PB、KX或IF麻醉下，我们通过甘露醇灌注的大脑半球的3+ Evans蓝染色获得了极好的BBBD作为证据。FD麻醉与心动过速相关，MF麻醉导致低血压;两者均显示伊文思蓝染色较差。放射性同位素输送到破坏的半球平均为0.80%的125 I-白蛋白，而对侧为0.03%，对照（盐水灌注）半球为0.06%。BBBD后99 mTc-葡庚糖酸盐递送量为给药剂量的0.49%，对侧为0.03%，对照半球为0.05%。在FD和MF组中，使心脏指数正常化的药理学操作导致3+伊文思蓝染色，并显著增加白蛋白和葡庚糖酸盐的递送。这项研究表明，这些特定的麻醉剂的心血管变化是重要的，在获得最佳的高渗BBBD。
To evaluate the effect of various anesthetic agents on hyperosmolar blood-brain barrier disruption (BBBD), Sprague-Dawley rats were given pentobarbital (PB), ketamine-xylazine (KX), isoflurane (IF), methoxylfurane (MF), or fentanyl-droperidol (FD) before intracarotid infusion of mannitol or saline. Physiological monitoring showed that the effects of mannitol infusion differed significantly from those of saline infusion and were associated with transient bradycardia, hypotension, metabolic acidosis, and electroencephalographic depression. With PB, KX, or IF anesthesia, we obtained excellent BBBD as evidence by 3+ Evans blue staining of the mannitol-infused cerebral hemisphere. FD anesthesia was associated with tachycardia and MF anesthesia resulted in hypotension; both showed poor Evens blue staining. Radioisotope delivery to the disrupted hemisphere averaged 0.80% of the administered 125I-albumin compared to 0.03% in the contralateral and 0.06% in control (saline-infused) hemispheres. 99mTc-glucoheptonate delivery measured 0.49% of the administered dose after BBBD, 0.03% contralaterally, and 0.05% in control hemispheres. Pharmacological manipulation to normalize the cardiac index in the FD and MF groups resulted in 3+ Evans blue staining and significantly increased delivery of albumin and glucoheptonate. This study suggests that the cardiovascular changes of these specific anesthetic agents are important in obtaining optimal hyperosmolar BBBD.