Remote homology identification of the Drosophila melanogaster ortholog of the RNA Polymerase I subunit Rpa34/POLR1G.
Remote homology identification of the Drosophila melanogaster ortholog of the RNA Polymerase I subunit Rpa34/POLR1G.
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DOI：
10.17912/micropub.biology.001107
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发表时间：
2024
影响因子：
--
通讯作者：
Knutson, Bruce
中科院分区：
文献类型：
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作者：
Palumbo, Ryan;Knutson, Bruce
高度保守的直向同源蛋白仅通过序列同源性就可以容易地鉴定，而保守性差的直向同源蛋白需要额外的结构信息来鉴定。所有果蝇直系同源的RNA聚合酶I，II和III亚基，除了一个已确定的序列同源性。在这里，我们确定CG 11076为果蝇中缺失的Rpa 34/POLR 1G直系同源物。同源性检测和二级结构分析表明，CG 11076与Rpa 34/POLR 1G具有高度的结构保守性，系统发育分析表明，这两个蛋白的亲缘关系较近。我们的工作强调了利用序列和结构来识别不同物种中高度不同的正向同源蛋白的重要性。
Highly conserved orthologous proteins are easily identified by sequence homology alone, whereas poorly conserved orthologs require additional structural information to be identified. All Drosophila orthologs of RNA polymerase I, II, and III subunits—except one—have been identified by sequence homology. Here, we identified CG11076 as the missing Rpa34/POLR1G ortholog in Drosophila . Remote homology detection and secondary structure analysis showed that CG11076 is predicted to have high structural conservation with Rpa34/POLR1G, and phylogenetic analysis demonstrated that these proteins are closely related. Our work underscores the importance of utilizing both sequence and structure to identify highly divergent orthologous proteins in different species.