Controlled delivery of bFGF remodeled vascular network in muscle flap and increased perfusion capacity via minor pedicle.
Controlled delivery of bFGF remodeled vascular network in muscle flap and increased perfusion capacity via minor pedicle.
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DOI：
10.1016/j.jss.2007.10.009
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发表时间：
2008-06
期刊：
影响因子：
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通讯作者：
Yoshihiro Yasuda;H. Koyama;Y. Tabata;Y. Fujihara;M. Oba;E. Uchinuma;T. Takato
中科院分区：
文献类型：
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作者：
Yoshihiro Yasuda;H. Koyama;Y. Tabata;Y. Fujihara;M. Oba;E. Uchinuma;T. Takato
背景浅层骨骼肌的血管蒂分为优势蒂和次要蒂。主要蒂可以可靠地为整个肌肉提供足够的循环，但次要蒂不能。因此，仅靠小蒂不可能抬高整个肌瓣。我们评估碱性成纤维细胞生长因子（bFGF）的递送是否会重塑肌瓣中的血管网络并通过小蒂增加灌注能力。 方法使用酸性明胶水凝胶微球的控释系统，将bFGF（50μg，FGF组）或磷酸盐缓冲盐水（PBS组）递送至含有单个优势蒂和小蒂的兔股薄肌。 7天后，将股薄肌提升为小蒂上的肌瓣，并用硅胶片包裹肌瓣，以避免周围组织的血管向内生长。术后7 d，评估皮瓣活力、显微血管造影和局部血流。结果FGF组皮瓣活力（73.6±20.5%）显着高于PBS组（19.7±23.5%）。皮瓣死后血管造影显示小蒂和优势蒂的远端血管网络之间存在明显的连通。 FGF组的血管造影评分和皮瓣局部血流量显着高于PBS组。结论将bFGF控制输送至兔股薄肌增加了小蒂的灌注能力，并且小蒂能够单独为整个肌肉提供足够的循环。
BACKGROUNDThe vascular pedicles of superficial skeletal muscles are classified as the dominant pedicle and the minor pedicle. The dominant pedicle can reliably provide adequate circulation to the entire muscle, but the minor pedicle cannot. Therefore, it is impossible to elevate an entire muscle flap based solely on the minor pedicle. We evaluated whether the delivery of basic fibroblast growth factor (bFGF) would remodel the vascular network in the muscle flap and increase the perfusion capacity via the minor pedicle.METHODSUsing a controlled delivery system with acidic gelatin hydrogel microspheres, bFGF (50 μg, FGF group) or phosphate-buffered saline (PBS group) was delivered to the rabbit gracilis muscle, which contained a single dominant pedicle and a minor pedicle. Seven days later, the gracilis muscle was elevated as a muscle flap on the minor pedicle, and the flap was wrapped with a silicone sheet to avoid vessel in-growth from surrounding tissue. At 7 d after operation, flap viability, microangiograms, and regional blood flow were evaluated.RESULTSFlap viability in the FGF group (73.6 ± 20.5%) was significantly higher than that in the PBS group (19.7 ± 23.5%). Postmortem angiograms of the flap showed obvious communications between the distal vascular networks of the minor and dominant pedicles. Angiographic score and regional blood flow of the flap were significantly higher in the FGF group than in the PBS group.CONCLUSIONSControlled delivery of bFGF to rabbit gracilis muscle increased the perfusion capacity via the minor pedicle, and the minor pedicle became able to solely provide sufficient circulation to the entire muscle.