Lectin-functionalized microchannels for characterizing pluripotent cells and early differentiation
Lectin-functionalized microchannels for characterizing pluripotent cells and early differentiation
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DOI：
10.1063/1.4719979
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发表时间：
2012-06-01
期刊：
影响因子：
3.2
通讯作者：
Murthy, Shashi K.
中科院分区：
文献类型：
--
作者：
Vickers, Dwayne A. L.;Kulik, Michael;Murthy, Shashi K.
胚胎干（ES）细胞能够增殖和分化形成三个胚胎胚层的细胞，即内胚层、中胚层和外胚层。人类 ES 细胞衍生物的利用需要能够在明确、高效和可扩展的系统中直接分化为特定谱系。需要更好的标记来识别早期分化。据报道，凝集素是常见干细胞标记物的有吸引力的替代品。它们已被用来根据细胞表面特定碳水化合物基团的呈现来识别、表征和分离各种细胞亚群。本文展示了凝集素包被的微流体通道中的简单粘附测定如何提供凝集素与 ES 和定形内胚层细胞相互作用的关键信息，从而跟踪早期分化。微流体方法结合了结合强度和细胞表面受体密度，而传统的流式细胞术仅结合了后者。这两种方法都经过检查并显示与微流体方法互补，提供更多的生物学相关信息。 (C) 2012 年美国物理研究所。 [http://dx.doi.org/10.1063/1.4719979]
Embryonic stem (ES) cells are capable of proliferating and differentiating to form cells of the three embryonic germ layers, namely, endoderm, mesoderm, and ectoderm. The utilization of human ES cell derivatives requires the ability to direct differentiation to specific lineages in defined, efficient, and scalable systems. Better markers are needed to identify early differentiation. Lectins have been reported as an attractive alternative to the common stem cell markers. They have been used to identify, characterize, and isolate various cell subpopulations on the basis of the presentation of specific carbohydrate groups on the cell surface. This article demonstrates how simple adhesion assays in lectin-coated microfluidic channels can provide key information on the interaction of lectins with ES and definitive endoderm cells and thereby track early differentiation. The microfluidic approach incorporates both binding strength and cell surface receptor density, whereas traditional flow cytometry only incorporates the latter. Both approaches are examined and shown to be complementary with the microfluidic approach providing more biologically relevant information. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4719979]