A SMALL MULTIGENE FAMILY ENCODES THE ROD-CORE LINKER POLYPEPTIDES OF ANABAENA SP PCC7120 PHYCOBILISOMES
A SMALL MULTIGENE FAMILY ENCODES THE ROD-CORE LINKER POLYPEPTIDES OF ANABAENA SP PCC7120 PHYCOBILISOMES
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DOI：
10.1016/0378-1119(91)90301-q
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发表时间：
1991-10-30
期刊：
影响因子：
3.5
通讯作者：
ZUBER, H
中科院分区：
文献类型：
--
作者：
BRYANT, DA;STIREWALT, VL;ZUBER, H
鱼腥藻(Anabaena sp.)PCC7120编码10个基因：5‘-cpcB-cpcA-cpcC-cpcD-cpcE-cpcF-cpcG1-cpcG2-cpcG3-cpcG4-3’.该操纵子的3‘端包括4个重复的基因，编码藻蓝蛋白(PC)相关的杆状连接多肽的藻胆体(PB)。这四个基因的产物在N端最相似，总体上有50%-61%的同源性和68%-76%的相似性。鱼腥藻的四种CPCG蛋白。PCC7120与聚球藻CPCG杆状核心连接蛋白的同源性为41%-47%，相似性为62%-65%。PCC7002。多肽的N-末端结构域也与PC、藻红蛋白和别藻蓝蛋白(AP)相关的其他类型的杆状连接多肽的保守结构域关系更远。N-末端氨基酸序列分析表明，其中3个杆状核心连接蛋白(CpcG1、CpcG2和CpcG4)存在于分离的PBS中。这些结果表明，以前提出的鱼腥藻PBS模型是可行的。是不正确的。建议对鱼腥藻PBS进行分类鉴定。有八个外周杆，每一个都通过特定的杆-核连接物(CPCG)多肽与核心的AP相互作用。
The cpc operon of Anabaena sp. PCC7120 is shown to encode ten genes: 5'-cpcB-cpcA-cpcC-cpcD-cpcE-cpcF-cpcG1-cpcG2-cpcG3-cpcG4-3'. The 3' portion of this operon includes four tandemly repeated genes encoding phycocyanin (PC)-associated, rod-core linker polypeptides of the phycobilisomes (PBS). The products of these four genes are most similar at their N termini, and overall are 50-61% identical and 68-76% similar to one another. The four CpcG proteins of Anabaena sp. PCC7120 are 41-47% identical and 62-65% similar to the single CpcG rod-core linker protein in Synechococcus sp. PCC7002. The N-terminal domains of the polypeptides are also more distantly related to the conserved domains of other types of rod-linker polypeptides associated with PC, phycoerythrin, and allophycocyanin (AP). Three of these rod-core linker proteins (CpcG1, CpcG2, and CpcG4) were demonstrated to occur in isolated PBS by N-terminal amino acid sequence analyses. These results indicate that previously proposed models for the PBS of Anabaena sp. are incorrect. It is suggested that the PBS of Anabaena sp. have eight peripheral rods, each of which interacts with the AP of the core via a specific rod-core linker (CpcG) polypeptide.