Interdependent nitric oxide and hydrogen peroxide independently regulate the coix seed oil-induced triterpene acid accumulation in Ganoderma lingzhi
Interdependent nitric oxide and hydrogen peroxide independently regulate the coix seed oil-induced triterpene acid accumulation in Ganoderma lingzhi
复制标题
相互依赖的一氧化氮和过氧化氢独立调节薏苡仁油诱导的灵芝三萜酸积累
DOI：
10.1080/00275514.2019.1615816
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发表时间：
2019-06-03
期刊：
影响因子：
2.8
通讯作者：
Liu, Gao-Qiang
中科院分区：
文献类型：
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作者：
Liu, Yong-Nan;Tong, Tian;Liu, Gao-Qiang
近年来，在培养基中添加外源植物油以促进几种药用蘑菇生物活性物质的产生已取得了一定的进展，但其作用机制尚不清楚。在本研究中，我们发现植物油薏苡仁油（CSO）可以诱导灵芝菌丝体中三萜酸（TA）的生物合成，并显著增加灵芝菌丝体细胞质中一氧化氮（NO）和过氧化氢（H2O2）的浓度。加入NO清除剂或H2O2清除剂可逆转CSO引起的TA生物合成的变化，加入NO清除剂或H2O2清除剂可分别降低CSO处理下胞质中H2O2或NO的浓度。此外，添加NO清除剂或H2O2清除剂可逆转TA的生物合成，H2O2或NO供体可分别挽救TA的生物合成。综上所述，我们的研究表明NO和H2O2都参与了G.灵芝。
Recent progress has been made in adding exogenous vegetable oils in culture media to promote bioactive metabolite production in several medicinal mushrooms, but the mechanism is still unclear. In this study, we found that the vegetable oil coix seed oil (CSO) could induce the biosynthesis of triterpene acids (TAs) and also significantly increase cytoplasmic nitric oxide (NO) and hydrogen peroxide (H2O2) concentrations in the mycelium of Ganoderma lingzhi. The change in TA biosynthesis caused by CSO could be reversed by adding NO scavenger or H2O2 scavenger, and adding NO scavenger or H2O2 scavenger resulted in the reduction of the cytoplasmic H2O2 or NO concentration under CSO treatment, respectively. Moreover, adding NO scavenger or H2O2 scavenger reversed TA biosynthesis, which could be rescued by H2O2 or NO donor, respectively. Taken together, our study indicated that both NO and H2O2 were involved in the regulation of TA biosynthesis, and CSO-activated NO and H2O2 were interdependent but independently regulated the TA biosynthesis under CSO treatment in G. lingzhi.