Attenuation effects of heparin-superoxide dismutase conjugate on bleomycin-induced lung fibrosis in vivo and radiation-induced inflammatory cytokine expression in vitro
Attenuation effects of heparin-superoxide dismutase conjugate on bleomycin-induced lung fibrosis in vivo and radiation-induced inflammatory cytokine expression in vitro
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肝素-超氧化物歧化酶缀合物对博来霉素诱导的体内肺纤维化和体外辐射诱导的炎症细胞因子表达的减弱作用
DOI：
10.1016/j.biopha.2008.04.009
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发表时间：
2009-08-01
影响因子：
7.5
通讯作者：
Cao, Jichao
中科院分区：
文献类型：
--
作者：
Liu, Jinfeng;Wang, Xuan;Cao, Jichao
本研究评估了肝素-超氧化物歧化酶偶联物（heparin-SOD）对博莱霉素诱导的肺纤维化的体内和体外炎症细胞因子表达的影响。为研究肝素- sod对肺纤维化的影响，采用博来霉素（BLM）处理小鼠腹腔注射肝素- sod，每天1次，每7 d测定肺组织中羟脯氨酸含量。利用组织病理学特征定量评估纤维化程度。结果显示肝素超氧化物歧化酶能抑制BLM诱导的肺纤维化病变，表现为BLM注入28天后肺羟脯氨酸含量降低，肺纤维化程度降低。体外对辐照3T3成纤维细胞表达的细胞因子水平的影响显示，肝素- sod显著降低转化生长因子- β 1和白细胞介素-1 β的水平。这些结果有力地证明肝素-超氧化物歧化酶可能在预防和治疗肺纤维化方面有用。(C) 2008 Elsevier Masson SAS。版权所有。
In this study, the effects of heparin-superoxide dismutase conjugate (heparin-SOD) on bleomycin-induced pulmonary fibrosis in vivo and on inflammatory cytokine expression in vitro were evaluated. To investigate the effects of heparin-SOD on pulmonary fibrosis, heparin-SOD was administered to bleomycin (BLM)-treated mice by intraperitoneal injection once a day and the hydroxyproline content in lung was determined per 7 days. The degree of fibrosis was assessed quantitatively using histopathologic features. The results showed that heparin-SOD inhibited BLM-induced lung fibrotic lesions as reflected by the decrease of lung hydroxyproline content and lung fibrosis grade 28 days after BLM instillation. The in vitro effects on the cytokine level expressed by irradiated 3T3 fibroblasts showed that heparin-SOD significantly lowered the levels of transforming growth factor-beta 1 and interleukin-1 beta. These results strongly demonstrated that heparin-SOD might be useful in the prevention and treatment of pulmonary fibrosis. (C) 2008 Elsevier Masson SAS. All rights reserved.