Design, synthesis and evaluation of ratiometric probes for hydrogencarbonate based on europium emission
Design, synthesis and evaluation of ratiometric probes for hydrogencarbonate based on europium emission
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DOI：
10.1039/b400734b
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发表时间：
2004-01-01
影响因子：
3.2
通讯作者：
Slater, R
中科院分区：
文献类型：
--
作者：
Bretonniere, Y;Cann, MJ;Slater, R
制备了一系列阳离子、两性离子和阴离子大环铕配合物，其中含有N或C连接的吖啶酮发色团，可以在390-410nm激发后敏化Eu发射。这些复合物中的每一种在生理pH值下选择性地结合碳酸氢盐，可逆结合通过Eu发射光谱的形式和相对强度的变化来表示。对碳酸氢盐的亲和力受整体复杂电荷的调节，并落在细胞内或细胞外分析所需的范围内。监测最多三个波长（例如618/588或618/702 nm）的Eu发射强度比，可以在乳酸盐、柠檬酸盐和磷酸盐等阴离子竞争的背景下推断出碳酸氢盐的溶液浓度。初步筛选显示，这些复合物对NIH-3T3细胞无毒，可以进入细胞内，这鼓励了进一步的研究。
A series of cationic, zwitterionic and anionic macrocyclic europium complexes has been prepared incorporating a N or C- linked acridone chromophore that allows sensitisation of Eu emission following excitation at 390-410nm. Each of these complexes selectively binds bicarbonate at physiological pH and reversible binding is signalled by a change in the form and relative intensity of the Eu emission spectrum. Affinity for bicarbonate is regulated by overall complex charge and falls within the range required for intracellular or extracellular analyses. Monitoring the ratio of the intensity of Eu emission at up to three wavelengths, e.g. 618/588 or 618/702 nm allows the solution concentration of bicarbonate to be deduced in a background of competing anions such as lactate, citrate and phosphate. Preliminary screens reveal the complexes to be non-toxic to NIH-3T3 cells and to be taken inside the cell, encouraging further study.