Engineering a 3D microfluidic culture platform for tumor-treating field application.
Engineering a 3D microfluidic culture platform for tumor-treating field application.
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DOI：
10.1038/srep26584
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发表时间：
2016-05-24
影响因子：
4.6
通讯作者：
Kamm RD
中科院分区：
文献类型：
--
作者：
Pavesi A;Adriani G;Tay A;Warkiani ME;Yeap WH;Wong SC;Kamm RD
当前癌症治疗的局限性突出了迫切需要一种更有效的治疗策略。一种很有希望的方法是使用交变电场；然而，参与破坏癌细胞周期的机制以及对非癌细胞的潜在不利影响必须澄清。在这项研究中，我们提出了一种具有嵌入式电极的新型微流控装置，可以将交变电场治疗应用于三维细胞外基质中的癌细胞。为了证明我们的系统在帮助设计和测试新的治疗方法方面的潜力，癌细胞和癌细胞聚集体被单独培养或与内皮细胞共培养。电场治疗后癌细胞的转移潜能降低。此外，与未处理的细胞相比，处理后的癌细胞的增殖速度较低，而内皮细胞的形态和增殖能力没有明显影响。这些结果表明，我们的新系统可用于快速筛选交变电场对体内微环境中癌症和正常细胞的影响，具有优化治疗方案和评估肿瘤治疗场治疗与化疗之间协同作用的潜力。
The limitations of current cancer therapies highlight the urgent need for a more effective therapeutic strategy. One promising approach uses an alternating electric field; however, the mechanisms involved in the disruption of the cancer cell cycle as well as the potential adverse effects on non-cancerous cells must be clarified. In this study, we present a novel microfluidic device with embedded electrodes that enables the application of an alternating electric field therapy to cancer cells in a 3D extracellular matrix. To demonstrate the potential of our system to aid in designing and testing new therapeutic approaches, cancer cells and cancer cell aggregates were cultured individually or co-cultured with endothelial cells. The metastatic potential of the cancer cells was reduced after electric field treatment. Moreover, the proliferation rate of the treated cancer cells was lower compared with that of the untreated cells, whereas the morphologies and proliferative capacities of the endothelial cells were not significantly affected. These results demonstrate that our novel system can be used to rapidly screen the effect of an alternating electric field on cancer and normal cells within an in vivo-like microenvironment with the potential to optimize treatment protocols and evaluate synergies between tumor-treating field treatment and chemotherapy.