Development, characterization, and anti-microbial efficacy of hydroxyapatite-chlorhexidine coatings produced by surface-induced mineralization.
Development, characterization, and anti-microbial efficacy of hydroxyapatite-chlorhexidine coatings produced by surface-induced mineralization.
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DOI：
10.1002/1097-4636(2000)53:4
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
Allison A. Campbell;Lin Song;X. Shari Li;Bradley J. Nelson;Craig Bottoni;Dan E. Brooks;E. Schuyler DeJong
中科院分区：
文献类型：
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作者：
Allison A. Campbell;Lin Song;X. Shari Li;Bradley J. Nelson;Craig Bottoni;Dan E. Brooks;E. Schuyler DeJong
采用表面诱导矿化（SIM）技术在外固定针上制备羟基磷灰石（HAP）涂层，涂层中加入抗菌剂氯己定。SIM过程涉及用有机官能团对基材进行表面改性，然后浸入过饱和磷酸钙水溶液中。X射线衍射谱证实了羟基磷灰石涂层的形成。通过在矿化循环之间将基材置于各种氯己定溶液中，将氯己定掺入涂层中。通过将涂层溶解到0.1M硝酸溶液中并使用UV光谱法在251 nm处测量氯己定浓度来测量总吸收。通过将涂覆的基材浸入盐水溶液中并测量作为时间函数的氯己定在231 nm处的UV吸光度来测量释放速率。结果显示，初始快速释放，随后是一段时间的缓慢缓释。使用金黄色葡萄球菌细胞培养物在体外评价HAP-洗必泰涂层的抗微生物功效。初始结果显示，与仅具有HAP的涂层相比，在氯己定/HAP涂层周围形成大的“抑制区”。这项初步工作清楚地表明，SIM HAP涂层具有在植入部位局部递送抗微生物剂（如氯己定）的巨大潜力，这可能大大降低外固定中发生的针道感染的发生率。
The surface-induced mineralization (SIM) technique was used to produce hydroxyapatite (HAP) coatings on external fixation pins with the antimicrobial agent, chlorhexidine, incorporated within the coating. The SIM process involved surface modification of the substrate with organic functional groups followed by immersion in aqueous supersaturated calcium phosphate solutions. X-ray diffraction spectra confirmed that hydroxyapatite coatings were formed. Chlorhexidine was incorporated into the coating by placing the substrate into various chlorhexidine solutions in between mineralization cycles. Total uptake was measured by dissolution of the coating into a 0.1 M nitric acid solution and measuring the chlorhexidine concentration using UV spectroscopy at 251 nm. Release rates were measured by submersion of coated substrates into saline solutions and measuring chlorhexidine UV absorbency at 231 nm as a function of time. Results show an initial rapid release followed by a period of slower sustained release. The anti-microbial efficacy of the HAP-chlorhexidine coatings was evaluated in vitro using a Staphylococcus aureus cell culture. Initial results show a large "inhibition zone" formed around the chlorhexidine/HAP coating vs. coatings with HAP only. This preliminary work clearly demonstrates that SIM HAP coatings have great potential to locally deliver antimicrobial agents such as chlorhexidine at implantation sites, which may greatly reduce the incidence of pin tract infection that occurs in external fixation.