Novel Carbon/PEDOT/PSS-Based Screen-Printed Biosensors for Acetylcholine Neurotransmitter and Acetylcholinesterase Detection in Human Serum
Novel Carbon/PEDOT/PSS-Based Screen-Printed Biosensors for Acetylcholine Neurotransmitter and Acetylcholinesterase Detection in Human Serum
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DOI：
10.3390/molecules24081539
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发表时间：
2019-04-02
期刊：
影响因子：
4.6
通讯作者：
Kamel, Ayman H.
中科院分区：
文献类型：
--
作者：
Ashmawy, Nashwa H.;Almehizia, Abdulrahman A.;Kamel, Ayman H.
采用聚（3，4-乙撑二氧噻吩）/聚（苯乙烯磺酸盐）（PEDOT/PSS）作为传感膜和电基底之间的固体接触，制备了新型可靠的电位离子选择性电极，用于乙酰胆碱（ACh）的生物测定。将PEDOT/PSS膜沉积在由陶瓷基底制成的固体碳丝网印刷平台上。在离子选择性电极（伊势）传感器膜中使用的选择性材料是乙酰胆碱四苯硼酸盐（ACh/TPB/PEDOT/PSS-ISE）（传感器I）和三乙酰基-β-环糊精（-CD/PEDOT/PSS-ISE）（传感器II）。该传感器显示出明显增强的能斯特响应，在pH 5下，在1.0 x 10（-6）-1 x 10（-3）和2.0 x 10（-6）-1.0 x 10（-3）M的动态线性范围内，对（ACh（+））离子的阳离子斜率为56.4 +/- 0.6和55.3 +/- 1.1 mV/decade，检测限为2.0 x 10（-7）。传感器I和II分别为3.2 × 10（-7）M。两种传感器的选择性行为也进行了测试和传感器显示出显着的高选择性对乙酰胆碱（+）不同常见的有机和无机阳离子。固体接触（SC）/ISE的电位响应的稳定性进行了评估，使用计时电位法，并与没有添加固体接触材料（PEDOT/PSS）制备的电极进行比较。这些具有成本效益的传感器具有更高的准确性、出色的重复性、良好的再现性、潜在的稳定性以及高选择性和灵敏度。该传感器还用于测量乙酰胆碱酯酶（AChE）的活性。乙酰胆碱（+）底物的初始水解速率与酶活力之间的线性关系为5.0 × 10 ~（-3）-5.2IU L ~（-1）。应用于人血清中乙酰胆碱的测定，并与标准比色法进行了比较。
New reliable and robust potentiometric ion-selective electrodes were fabricated using poly(3,4-ethylenedioxythiophene)/poly(styrenesulfonate) (PEDOT/PSS) as the solid contact between the sensing membrane and electrical substrate for an acetylcholine (ACh) bioassay. A film of PEDOT/PSS was deposited on a solid carbon screen-printed platform made from ceramic substrate. The selective materials used in the ion-selective electrode (ISE) sensor membrane were acetylcholinium tetraphenylborate (ACh/TPB/PEDOT/PSS-ISE) (sensor I) and triacetyl--cyclodextrin (-CD/PEDOT/PSS-ISE) (sensor II). The sensors revealed clear enhanced Nernstian response with a cationic slope 56.4 +/- 0.6 and 55.3 +/- 1.1 mV/decade toward (ACh(+)) ions over the dynamic linear range 1.0 x 10(-6)-1 x 10(-3) and 2.0 x 10(-6)-1.0 x 10(-3) M at pH 5 with limits of detection 2.0 x 10(-7) and 3.2 x 10(-7) M for sensors I and II, respectively. The selectivity behavior of both sensors was also tested and the sensors showed a significant high selectivity toward ACh(+) over different common organic and inorganic cations. The stability of the potential response for the solid-contact (SC)/ISEs was evaluated using a chronopotentiometric method and compared with that of electrodes prepared without adding the solid-contact material (PEDOT/PSS). Enhanced accuracy, excellent repeatability, good reproducibility, potential stability, and high selectivity and sensitivity were introduced by these cost-effective sensors. The sensors were also used to measure the activity of acetylcholinesterase (AChE). A linear plot between the initial rate of the hydrolysis of ACh(+) substrate and enzyme activity held 5.0 x 10(-3)-5.2 IU L-1 of AChE enzyme. Application to acetylcholine determination in human serum was done and the results were compared with the standard colorimetric method.