Aerosolized Silver Nanoparticles in the Rat Lung and Pulmonary Responses over Time.
Aerosolized Silver Nanoparticles in the Rat Lung and Pulmonary Responses over Time.
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DOI：
10.1177/0192623316629804
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发表时间：
2016-07
影响因子：
1.5
通讯作者：
Pinkerton KE
中科院分区：
文献类型：
--
作者：
Silva RM;Anderson DS;Peake J;Edwards PC;Patchin ES;Guo T;Gordon T;Chen LC;Sun X;Van Winkle LS;Pinkerton KE
银纳米颗粒 (Ag NP) 的生产方法正在开发和完善，通过涉及银盐溶液、溶剂和封端剂的化学反应来控制颗粒形成，生产更均匀的银纳米颗粒。这些化学反应物通常作为污染物和/或银纳米颗粒涂层存在，这可能会改变它们在体内的相互作用。为了确定柠檬酸盐包被的银纳米颗粒对肺部的影响，将 Sprague-Dawley 大鼠仅用鼻子暴露于雾化的银纳米颗粒（20 nm [C20] 或 110 nm [C110] Ag NP）中六小时。在暴露后 1、7、21 和 56 天获取支气管肺泡灌洗液 (BALF) 和肺组织进行分析。与柠檬酸盐缓冲液对照相比，吸入 Ag NP 会产生显着的炎症和细胞毒性反应，并在 BALF 细胞和上清液中进行测量。第 7 天，BALF 中的总细胞、蛋白质和乳酸脱氢酶显着升高，与对照相比，在 C20 或 C110 暴露后观察到组织病理学峰值。第 21 天，与 C110 相比，C20 暴露后 BALF PMN 和组织炎症明显加重。到第 56 天，暴露于 Ag NP 的动物的炎症得到解决。总体而言，结果表明吸入 C20 或 C110 后会出现延迟、短暂的炎症和细胞毒性作用，并且接触 C20 后可能会产生更大的反应。
Silver nanoparticle (Ag NP) production methods are being developed and refined to produce more uniform Ag NPs through chemical reactions involving silver salt solutions, solvents, and capping agents to control particle formation. These chemical reactants are often present as contaminants and/or coatings on the Ag NPs, which could alter their interactions in vivo. To determine pulmonary effects of citrate-coated Ag NPs, Sprague-Dawley rats were exposed once nose-only to aerosolized Ag NPs (20 nm [C20] or 110 nm [C110] Ag NPs) for six hours. Bronchoalveolar lavage fluid (BALF) and lung tissues were obtained at 1, 7, 21, and 56 days post exposure for analyses. Inhalation of Ag NPs, versus citrate buffer control, produced significant inflammatory and cytotoxic responses that were measured in BALF cells and supernatant. At Day 7, total cells, protein, and lactate dehydrogenase were significantly elevated in BALF, and peak histopathology was noted after C20 or C110 exposure versus control. At Day 21, BALF PMNs and tissue inflammation was significantly greater after C20 versus C110 exposure. By Day 56, inflammation was resolved in Ag NP-exposed animals. Overall, results suggest delayed, short-lived inflammatory and cytotoxic effects following C20 or C110 inhalation and potential for greater responses following C20 exposure.