Fig1 facilitates calcium influx and localizes to membranes destined to undergo fusion during mating in Candida albicans.
Fig1 facilitates calcium influx and localizes to membranes destined to undergo fusion during mating in Candida albicans.
复制标题
Fig1 促进钙内流，并定位于白色念珠菌交配过程中发生融合的膜。
作者：
Yang M
Few mating-regulated genes have been characterized inCandida albicans. C. albicans FIG1(CaFIG1) is a fungus-specific and mating-induced gene encoding a putative 4-transmembrane domain protein that shares sequence similarities with members of the claudin superfamily. In Saccharomyces cerevisiae, Fig1 is required for shmoo fusion and is upregulated in response to mating pheromones. Expression of CaFIG1was also strongly activated in the presence of cells of the opposite mating type. CaFig1-green fluorescent protein (GFP) was visible only during the mating response, when it localized predominantly to the plasma membrane and perinuclear zone in mating projections and daughter cells. At the plasma membrane, CaFig1-GFP was visualized as discontinuous zones, but the distribution of perinuclear CaFig1-GFP was homogeneous. Exposure to pheromone induced a 5-fold increase in Ca2+uptake in mating-competent opaque cells. Uptake was reduced substantially in thefig1Δ null mutant. CaFig1 is therefore involved in Ca2+influx and localizes to membranes that are destined to undergo fusion during mating.
